Evidence map›Paper›PMID 38351444›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells (SSCs).

Janmejay Hingu, Guillermo Galdon, Nicholas A Deebel, Hooman Sadri-Ardekani

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 synthesis or guideline pooled it.

  1. Guideline
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Janmejay HinguDepartment of Urology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Guillermo GaldonWake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Nicholas A DeebelDepartment of Urology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Hooman Sadri-ArdekaniDepartment of Urology, Wake Forest University School of Medicine, Winston-Salem, NC, USA. hsadri@wakehealth.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Preservation of human spermatogonial stem cells (SSCs) may be suitable for young male patients at risk of male infertility due to various causes, such as gonadotoxic treatment or genetic diseases. With optimal cryopreservation, cell viability can be retained to reestablish spermatogenesis in the future through autologous transplantation or in vitro differentiation of SSCs. This protocol outlines techniques to optimize the SSCs isolation and in vitro culture. With particular emphasis on the microscopic characteristics encountered, this protocol outlines a broader approach to processing tissues with varying morphologies among patients.

Indexed as

Adult Germline Stem CellsInfertility, MaleCryopreservationHumansMaleSpermatogenesisSpermatogoniaTestisIsolationMale infertilityPropagationSpermatogonial stem cell (SSC)

Identifiers

PMID38351444

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.