Evidence map›Paper›PMID 38351231›Full record

ArticleNature nanotechnology2024

A non-FRET DNA reporter that changes fluorescence colour upon nuclease digestion.

Soonwoo Hong, Jada N Walker, Aaron T Luong, Jonathan Mathews, Samuel W J Shields, Yu-An Kuo, Yuan-I Chen, Trung Duc Nguyen, Yujie He, Anh-Thu Nguyen and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
4.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 39 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Soonwoo HongDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0000-0002-7618-9052
Jada N WalkerDepartment of Chemistry, The University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0000-0001-6233-3124
Aaron T LuongDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Jonathan MathewsDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0009-0005-2813-9519
Samuel W J ShieldsDepartment of Chemistry, The University of Texas at Austin, Austin, TX, USA.
Yu-An KuoDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Yuan-I ChenDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Trung Duc NguyenDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Yujie HeDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.ORCID http://orcid.org/0000-0002-6984-3052
Anh-Thu NguyenDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA.
Madhav L GhimireDepartment of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA.ORCID http://orcid.org/0009-0006-6896-3112
Min Jun KimDepartment of Mechanical Engineering, Southern Methodist University, Dallas, TX, USA.ORCID http://orcid.org/0000-0002-0819-1644
Jennifer S BrodbeltDepartment of Chemistry, The University of Texas at Austin, Austin, TX, USA.
Hsin-Chih YehDepartment of Biomedical Engineering, The University of Texas at Austin, Austin, TX, USA. tim.yeh@austin.utexas.edu.ORCID http://orcid.org/0000-0001-6654-5626
The University of Texas at Austin · USSouthern Methodist University · US

Funding

Developing an ultrafast fluorescence lifetime imaging ophthalmoscopy system for retinal imagingR21EY033106 · NEI · UNIVERSITY OF TEXAS AT AUSTIN · PI YEH, HSIN-CHIH · 2021 to 2022
$399k
National Science Foundation (NSF) 2029266National Science Foundation (NSF) 2041340National Science Foundation (NSF) 2041345NEI NIH HHS R21 EY033106U.S. Department of Health & Human Services | National Institutes of Health (NIH) 033106
6 · The paper itself

Abstract

Fluorescence resonance energy transfer (FRET) reporters are commonly used in the final stages of nucleic acid amplification tests to indicate the presence of nucleic acid targets, where fluorescence is restored by nucleases that cleave the FRET reporters. However, the need for dual labelling and purification during manufacturing contributes to the high cost of FRET reporters. Here we demonstrate a low-cost silver nanocluster reporter that does not rely on FRET as the on/off switching mechanism, but rather on a cluster transformation process that leads to fluorescence color change upon nuclease digestion. Notably, a 90 nm red shift in emission is observed upon reporter cleavage, a result unattainable by a simple donor-quencher FRET reporter. Electrospray ionization-mass spectrometry results suggest that the stoichiometric change of the silver nanoclusters from Ag

Indexed as

DNAFluorescence Resonance Energy TransferSilverColorFluorescenceMetal NanoparticlesNanostructuresDNASilver

Identifiers

PMID38351231
PMCPMC11864325
OpenAlexW4391779828

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.