Automatic enhancement preprocessing for segmentation of low quality cell images.

We present a novel automatic preprocessing and ensemble learning technique for the segmentation of low-quality cell images. Capturing cells subjected to intense light is challenging due to their vulnerability to light-induced cell death. Consequently, microscopic cell images tend to be of low quality and it causes low accuracy for semantic segmentation. This problem can not be satisfactorily solved by classical image preprocessing methods. Therefore, we propose a novel approach of automatic enhancement preprocessing (AEP), which translates an input image into images that are easy to recognize by deep learning. AEP is composed of two deep neural networks, and the penultimate feature maps of the first network are employed as filters to translate an input image with low quality into images that are easily classified by deep learning. Additionally, we propose an automatic weighted ensemble learning (AWEL), which combines the multiple segmentation results. Since the second network predicts segmentation results corresponding to each translated input image, multiple segmentation results can be aggregated by automatically determining suitable weights. Experiments on two types of cell image segmentation confirmed that AEP can translate low-quality cell images into images that are easy to segment and that segmentation accuracy improves using AWEL.