ArticleApplied and environmental microbiology2024
A direct PCR approach with low-biomass insert opens new horizons for molecular sciences on cryptogam communities.
Article in Applied and environmental microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 9 citations in OpenAlex.
- Linking Biocrust Architecture and Dispersal: Reproductive Ecology of Lichens of the Grit Crust in the Coastal Atacama Desert.Environmental microbiology reports · 2026Article
- Fog, Symbiosis, and Survival: The Ecological Architecture of the Grit Crust From the Atacama Desert Represents a Lichen Holobiome Rather Than a Soil Microbiome.Environmental microbiology · 2026Article
- A glimpse into darkness: Diversity of culturable cyanobacteria, green algae and fungi from subaerial cave biofilms.Journal of phycology · 2025Article
- A hitchhiker's guide to modern, practical cyanobacterial taxonomy.Journal of phycology · 2025Article
- Four novel taxa of cyanobacteria from a unique thermal cave habitat in Vromoner Canyon, Albania.Journal of phycology · 2025Article
- The Desert in Bottles: The Culturable Microbiome of the Atacama Desert's Grit Crust.Environmental microbiology reports · 2025Article
- Microbiome Dynamics in Early Life Stages of the Precious Mediterranean Red Coral Corallium rubrum.Environmental microbiology reports · 2025Article
- Hard shell, soft blue-green core: Ecology, processes, and modern applications of calcification in terrestrial cyanobacteria.iScience · 2024Review
- High-quality genome of a novel Thermosynechococcaceae species from Namibia and characterization of its protein expression patterns at elevated temperatures.MicrobiologyOpen · 2024Article
- The symbiotic alga Trebouxia fuels a coherent soil ecosystem on the landscape scale in the Atacama Desert.Environmental microbiome · 2024Article
- The underestimated fraction: diversity, challenges and novel insights into unicellular cyanobionts of lichens.ISME communications · 2024Review
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Molecular sequence data have transformed research on cryptogams (e.g., lichens, microalgae, fungi, and symbionts thereof) but methods are still strongly hampered by the small size and intermingled growth of the target organisms, poor cultivability and detrimental effects of their secondary metabolites. Here, we aim to showcase examples on which a modified direct PCR approach for diverse aspects of molecular work on environmental samples concerning biocrusts, biofilms, and cryptogams gives new options for the research community. Unlike traditional approaches, this methodology only requires biomass equivalent to colonies and fragments of 0.2 mm in diameter, which can be picked directly from the environmental sample, and includes a quick DNA lysis followed by a standardized PCR cycle that allows co-cycling of various organisms/target regions in the same run. We demonstrate that this modified method can (i) amplify the most widely used taxonomic gene regions and those used for applied and environmental sciences from single colonies and filaments of free-living cyanobacteria, bryophytes, fungi, and lichens, including their mycobionts, chlorobionts, and cyanobionts from both isolates and
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