Evidence map›Paper›PMID 38347914›Full record

ArticleFrontiers in bioengineering and biotechnology2024

A simple and inexpensive laser dissection of fasciculated axons from motor nerve organoids.

Yasuhiro Ikegami, Tomoya Duenki, Ikuma Arakaki, Ryo Sakai, Tatsuya Osaki, Satoshi Ashihara, Tsuyoshi Furushima, Yoshiho Ikeuchi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yasuhiro IkegamiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Tomoya DuenkiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Ikuma ArakakiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Ryo SakaiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Tatsuya OsakiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Satoshi AshiharaInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Tsuyoshi FurushimaInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Yoshiho IkeuchiInstitute of Industrial Science, The University of Tokyo, Tokyo, Japan.
The University of Tokyo · JPLaboratory for Integrated Micro-Mechatronic Systems · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Motor nerve organoids could be generated by culturing a spheroid of motor neurons differentiated from human induced pluripotent stem (iPS) cells within a polydimethylsiloxane (PDMS) chip which guides direction and fasciculation of axons extended from the spheroid. To isolate axon bundles from motor nerve organoids, we developed a rapid laser dissection method based on localized photothermal combustion. By illuminating a blue laser on a black mark on the culture device using a dry-erase marker, we induced highly localized heating near the axon bundles. Moving the laser enabled spatial control over the local heating and severing of axon bundles. This laser dissection requires a black mark, as other colors did not produce the same localized heating effect. A CO

Indexed as

axonslaserorganoidsPDMStissue engineering

Identifiers

PMID38347914
PMCPMC10859526
OpenAlexW4391320756

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.