Evidence map›Paper›PMID 38347430›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Mechanical Characterization of Mucus on Intestinal Tissues by Atomic Force Microscopy.

Momoka Horikiri, Mugen Taniguchi, Hiroshi Y Yoshikawa, Ryu Okumura, Takahisa Matsuzaki

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
10.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Momoka HorikiriDepartment of Applied Physics, Graduate School of Engineering, Osaka University, Osaka, Japan.
Mugen TaniguchiDepartment of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Hiroshi Y YoshikawaDepartment of Applied Physics, Graduate School of Engineering, Osaka University, Osaka, Japan.
Ryu OkumuraDepartment of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, Japan. okumura@ongene.med.osaka-u.ac.jp.
Takahisa MatsuzakiDepartment of Applied Physics, Graduate School of Engineering, Osaka University, Osaka, Japan. matsuzaki@ap.eng.osaka-u.ac.jp.
The University of Osaka · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mucus is part of the innate immune system that defends the mucosa against microbiota and other infectious threats. The mechanical characteristics of mucus, such as viscosity, elasticity, and lubricity, are critically involved in its barrier function. However, assessing the mechanical properties of mucus remains challenging because of technical limitations. Thus, a new approach that characterizes the mechanical properties of mucus on colonic tissues needs to be developed. Here, we describe a novel strategy to characterize the ex vivo mechanical properties of mucus on colonic tissues using atomic force microscopy. This description includes the preparation of the mouse colon sample, AFM calibration, and determining the elasticity (Young's modulus, E [kPa]) of the mucus layer in the colon.

Indexed as

Microscopy, Atomic ForceAnimalsElasticityElastic ModulusMiceAtomic force microscopy (AFM)ElasticityMucus layerYoung’s modulus (E kPa)

Identifiers

PMID38347430
OpenAlexW4391789224

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.