ArticleScientific reports2024
Adaptation and validation of a quantitative vanA/vanB DNA screening assay on a high-throughput PCR system.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Article
- Evaluation of sample pooling using Xpert Carba-R and XpertMicrobiology spectrum · 2025Article
- Automated molecular detection of the vancomycin resistance genes vanA and vanB using the geneLEAD VIII platform.Access microbiology · 2025Article
- Present and Future Applications of Digital PCR in Infectious Diseases Diagnosis.Diagnostics (Basel, Switzerland) · 2024Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vancomycin resistant enterococci (VRE) are a leading cause of ICU-acquired bloodstream infections in Europe. The bacterial load in enteral colonization may be associated with a higher probability of transmission. Here, we aimed to establish a quantitative vanA/vanB DNA real-time PCR assay on a high-throughput system. Limits of detection (LOD), linear range and precision were determined using serial bacterial dilutions. LOD was 46.9 digital copies (dcp)/ml for vanA and 60.8 dcp/ml for vanB. The assay showed excellent linearity between 4.7 × 10
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Registered trials
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