Evidence map›Paper›PMID 38346245›Full record

ReviewAnnual review of biophysics2024

Next-Generation Genetically Encoded Fluorescent Biosensors Illuminate Cell Signaling and Metabolism.

Michelle S Frei, Sohum Mehta, Jin Zhang

Open access · hybridAbstract readReview
In one paragraph

Review in Annual review of biophysics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
9.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Michelle S FreiDepartment of Pharmacology, University of California San Diego, La Jolla, California, USA; email: msfrei@health.ucsd.edu, som010@health.ucsd.edu, jzhang32@health.ucsd.edu.
Sohum MehtaDepartment of Pharmacology, University of California San Diego, La Jolla, California, USA; email: msfrei@health.ucsd.edu, som010@health.ucsd.edu, jzhang32@health.ucsd.edu.
Jin ZhangDepartment of Pharmacology, University of California San Diego, La Jolla, California, USA; email: msfrei@health.ucsd.edu, som010@health.ucsd.edu, jzhang32@health.ucsd.edu.
University of California, San Diego · US

Funding

Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2M
Mechanisms of Compartmentalized cAMP SignalingR01DK073368 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2006 to 2026
$7.6M
Ultrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3M
AMPK Regulation of ACE2 in Endothelial Health and DiseaseR01HL162302 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SHYY, JOHN YJ, ZHANG, JIN · 2022 to 2025
$2.7M
Development of kinase biosensors for multiplex neuronal imaging of signaling pathways in behaving miceRF1MH126707 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L, ZHANG, JIN · 2022 to 2022
$2.4M
Signal Transduction by PI3K/mTORR01DE030497 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GUTKIND, JORGE SILVIO, ZHANG, JIN · 2021 to 2025
$2.4M
NCI NIH HHS R01 CA262815NCI NIH HHS R35 CA197622NHLBI NIH HHS R01 HL162302NIDCR NIH HHS R01 DE030497NIDDK NIH HHS R01 DK073368NIMH NIH HHS RF1 MH126707
6 · The paper itself

Abstract

Genetically encoded fluorescent biosensors have revolutionized the study of cell signaling and metabolism, as they allow for live-cell measurements with high spatiotemporal resolution. This success has spurred the development of tailor-made biosensors that enable the study of dynamic phenomena on different timescales and length scales. In this review, we discuss different approaches to enhancing and developing new biosensors. We summarize the technologies used to gain structural insights into biosensor design and comment on useful screening technologies. Furthermore, we give an overview of different applications where biosensors have led to key advances over recent years. Finally, we give our perspective on where future work is bound to make a large impact.

Indexed as

Biosensing TechniquesSignal TransductionAnimalsFluorescent DyesHumansFluorescent Dyesbiosensor designbiosensor multiplexingfluorescence microscopyhybrid biosensorsin vivo imagingsuper-resolution microscopy

Identifiers

PMID38346245
PMCPMC11786609
OpenAlexW4391741634

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.