Evidence map›Paper›PMID 38345319›Full record

ArticleDevelopment (Cambridge, England)2024

Sall4 regulates posterior trunk mesoderm development by promoting mesodermal gene expression and repressing neural genes in the mesoderm.

Matthew P Pappas, Hiroko Kawakami, Dylan Corcoran, Katherine Q Chen, Earl Parker Scott, Julia Wong, Micah D Gearhart, Ryuichi Nishinakamura, Yasushi Nakagawa, Yasuhiko Kawakami

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Matthew P PappasDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Hiroko KawakamiDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Dylan CorcoranDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Katherine Q ChenDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-0982-9179
Earl Parker ScottDepartment of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA.
Julia WongDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.
Micah D GearhartDevelopmental Biology Center, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-9873-1930
Ryuichi NishinakamuraDepartment of Kidney Development, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto 860-0811, Japan.
Yasushi NakagawaStem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0003-4876-5718
Yasuhiko KawakamiDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-0043-9705
University of Minnesota · USKumamoto University · JP

Funding

Genetic Regulation of Progenitor Cells in Appendicular Skeletal DevelopmentR01AR064195 · NIAMS · UNIVERSITY OF MINNESOTA · PI KAWAKAMI, YASUHIKO · 2013 to 2022
$3.3M
NIAMS NIH HHS R01 AR064195NIH HHS R01AR064195
6 · The paper itself

Abstract

The trunk axial skeleton develops from paraxial mesoderm cells. Our recent study demonstrated that conditional knockout of the stem cell factor Sall4 in mice by TCre caused tail truncation and a disorganized axial skeleton posterior to the lumbar level. Based on this phenotype, we hypothesized that, in addition to the previously reported role of Sall4 in neuromesodermal progenitors, Sall4 is involved in the development of the paraxial mesoderm tissue. Analysis of gene expression and SALL4 binding suggests that Sall4 directly or indirectly regulates genes involved in presomitic mesoderm differentiation, somite formation and somite differentiation. Furthermore, ATAC-seq in TCre; Sall4 mutant posterior trunk mesoderm shows that Sall4 knockout reduces chromatin accessibility. We found that Sall4-dependent open chromatin status drives activation and repression of WNT signaling activators and repressors, respectively, to promote WNT signaling. Moreover, footprinting analysis of ATAC-seq data suggests that Sall4-dependent chromatin accessibility facilitates CTCF binding, which contributes to the repression of neural genes within the mesoderm. This study unveils multiple mechanisms by which Sall4 regulates paraxial mesoderm development by directing activation of mesodermal genes and repression of neural genes.

Indexed as

DNA-Binding ProteinsGene Expression Regulation, DevelopmentalMesodermTranscription FactorsAnimalsCell DifferentiationChromatinGene ExpressionMiceSomitesChromatinDNA-Binding ProteinsSall4 protein, mouseTranscription FactorsChromatin accessibilityPosterior trunkPresomitic mesodermSall4SomitesWNT signaling

Identifiers

PMID38345319
PMCPMC10946440
OpenAlexW4391754750

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.