Modern approaches to improving phase contrast electron microscopy.

Jeremy J Axelrod, Jessie T Zhang, Petar N Petrov, Robert M Glaeser, Holger Mȕller

Open access · greenAbstract readPreprint

In one paragraph

Article in ArXiv, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

9.9field-weighted citation impact, top 2% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 19 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Updated by
Modern approaches to improving phase contrast electron microscopy.2024

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jeremy J AxelrodDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.

Jessie T ZhangDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.

Petar N PetrovDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.

Robert M GlaeserDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.

Holger MȕllerDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.

Lawrence Berkeley National Laboratory · USUniversity of California, Berkeley · US

Funding

Development of Laser-Based Phase Contrast for Biological Electron MicroscopyR01GM126011 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI MUELLER, HOLGER · 2017 to 2024

$3.9M

Investigating MHC-I downregulation by HIV-1 Nef using cryo-electron tomography with laser-based phase contrastF32GM149186 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI PETROV, PETAR NIKOLOV · 2023 to 2023

$70k

NIGMS NIH HHS F32 GM149186NIGMS NIH HHS R01 GM126011

6 · The paper itself

Abstract

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a phase shift to the unscattered part of the electron beam. Many approaches have been investigated, including the ponderomotive interaction between light and electrons. We review the recent successes achieved with this method in high-resolution, single-particle cryo-EM. We also review the status of using pulsed or near-field enhanced laser light as alternatives, along with approaches that use scanning transmission electron microscopy (STEM) with a segmented detector rather than a phase plate.

Identifiers

PMID38344223

PMCPMC10854270

OpenAlexW4393176588

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.