Evidence map›Paper›PMID 38341572›Full record

ArticleMicrobial cell factories2024

Development and characterization of a novel nanobody with SRMV neutralizing activity.

Miao Sun, Changjiang Wang, Huaye Luo, Yanfei Chen, Guanggang Qu, Jian Chen, Ling Li, Min Zhang, Qinghong Xue

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Miao Sun *Department of Viral Biologics, China Institute of Veterinary Drug Control, Beijing, China.
Changjiang Wang *Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, China.
Huaye LuoShanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, China.
Yanfei ChenDepartment of Viral Biologics, China Institute of Veterinary Drug Control, Beijing, China.
Guanggang QuShandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, China.
Jian ChenDepartment of Viral Biologics, China Institute of Veterinary Drug Control, Beijing, China.
Ling LiDepartment of Viral Biologics, China Institute of Veterinary Drug Control, Beijing, China.
Min ZhangTech-Bank Food Corporation Limited, Nanjing, China.
Qinghong XueDepartment of Viral Biologics, China Institute of Veterinary Drug Control, Beijing, China. 18811026336@163.com.

Funding

National Natural Science Foundation of China 32072895Natural Fund of Shandong Province，China ZR2020MC186
6 · The paper itself

Abstract

Peste des petits ruminants (PPR) is an acute, contact infectious disease caused by the small ruminant morbillivirus (SRMV), and its morbidity in goats and sheep can be up to 100% with significant mortality. Nanobody generated from camelid animals such as alpaca has attracted wide attention because of its unique advantages compared with conventional antibodies. The main objective of this study was to produce specific nanobodies against SRMV and identify its characteristics. To obtain the coding gene of SRMV-specific nanobodies, we first constructed an immune phage-displayed library from the VHH repertoire of alpaca that was immunized with SRMV-F and -H proteins. By using phage display technology, the target antigen-specific VHHs can be obtained after four consecutive rounds of biopanning. Results showed that the size of this VHH library was 2.26 × 10

Indexed as

BacteriophagesCamelids, New WorldPeste-des-Petits-RuminantsPeste-des-petits-ruminants virusSingle-Domain AntibodiesAnimalsAntibodiesAntigensEscherichia coliGoatsSheepAntibodiesAntigensSingle-Domain AntibodiesFusion proteinHemagglutinin proteinNanobodySmall ruminant morbillivirus (SRMV)

Identifiers

PMID38341572
PMCPMC10858559

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.