Evidence map›Paper›PMID 38340191›Full record

ArticlePlant cell reports2024

Development of PEG-mediated genetic transformation and gene editing system of Bryum argenteum as an abiotic stress tolerance model plant.

Fengjun Leng, Guiwei Zhou, Ruoyuan Shi, Chengyang Liu, Yirui Lin, Xinqiang Yu, Yanhua Zhang, Xiangxi He, Zhu Liu, Mingming Sun and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Plant cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Fengjun LengKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Guiwei ZhouKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Ruoyuan ShiKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Chengyang LiuKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Yirui LinKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Xinqiang YuKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Yanhua ZhangKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Xiangxi HeKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Zhu LiuKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Mingming SunLaboratory for Micro-Sized Functional Materials, College of Elementary Education, Capital Normal University, Beijing, 100048, China.
Fang BaoKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China. 5838@cnu.edu.cn.
Yong HuKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China. 5247@cnu.edu.cn.ORCID http://orcid.org/0009-0003-7665-3542
Yikun HeKey Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government, and College of Life Sciences, Capital Normal University, Beijing, 100048, China.
Beijing Municipal Government · CNBeijing Normal University · CNCapital Normal University · CN

Funding

General Program of National Natural Science Foundation of China No.31970658 Y.HeMajor Research Plan of National Nature Science Foundation of China 91631109 to Y.He
6 · The paper itself

Abstract

key messageTo establish a sterile culture system and protoplast regeneration system for Bryum argenteum, and to establish and apply CRISPR/Cas9 system in Bryum argenteum. Bryum argenteum is a fascinating, cosmopolitan, and versatile moss species that thrives in various disturbed environments. Because of its comprehensive tolerance to the desiccation, high UV and extreme temperatures, it is emerging as a model moss for studying the molecular mechanisms underlying plant responses to abiotic stresses. However, the lack of basic tools such as gene transformation and targeted genome modification has hindered the understanding of the molecular mechanisms underlying the survival of B. argenteum in different environments. Here, we reported the protonema of B. argenteum can survive up to 95.4% water loss. In addition, the genome size of B. argenteum is approximately 313 Mb by kmer analysis, which is smaller than the previously reported 700 Mb. We also developed a simple method for protonema induction and an efficient protoplast isolation and regeneration protocol for B. argenteum. Furthermore, we established a PEG-mediated protoplast transient transfection and stable transformation system for B. argenteum. Two homologues of ABI3(ABA-INSENSITIVE 3) gene were successfully cloned from B. argenteum. To further investigate the function of the ABI3 gene in B. argenteum, we used the CRISPR/Cas9 genetic editing system to target the BaABI3A and BaABI3B gene in B. argenteum protoplasts. This resulted in mutagenesis at the target in about 2-5% of the regenerated plants. The isolated abi3a and abi3b mutants exhibited increased sensitivity to desiccation, suggesting that BaABI3A and BaABI3B play redundant roles in desiccation stress. Overall, our results provide a rapid and simple approach for molecular genetics in B. argenteum. This study contributes to a better understanding of the molecular mechanisms of plant adaptation to extreme environmental.

Indexed as

BryophytaBryopsidaCRISPR-Cas SystemsGene EditingProtoplastsStress, PhysiologicalTransformation, GeneticABI3Bryum argenteumCRISPR/Cas9 systemPEG-mediated transformationProtoplasts

Identifiers

PMID38340191
OpenAlexW4391718726

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.