ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2024
Cold storage and cryopreservation methods for spermatozoa of the sea urchins Lytechinus pictus and Strongylocentrotus purpuratus.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Cryopreservation of sea urchin (Lytechinus pictus) embryos and development through metamorphosis.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- The tuxedo sea urchinbioRxiv : the preprint server for biology · 2026Article
- Successful cryopreservation of marine invertebrates immune cells enables long-term studies of common octopus,Frontiers in immunology · 2025Article
- Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus.Development (Cambridge, England) · 2024Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundSea urchins have contributed greatly to knowledge of fertilization, embryogenesis, and cell biology. However, until now, they have not been genetic model organisms because of their long generation times and lack of tools for husbandry and gene manipulation. We recently established the sea urchin Lytechinus pictus, as a multigenerational model Echinoderm, because of its relatively short generation time of 4-6 months and ease of laboratory culture. To take full advantage of this new multigenerational species, methods are needed to biobank and share genetically modified L. pictus sperm.
resultsHere, we describe a method, based on sperm ion physiology that maintains L. pictus and Strongylocentrotus purpuratus sperm fertilizable for at least 5-10 weeks when stored at 0°C. We also describe a new method to cryopreserve sperm of both species. Sperm of both species can be frozen and thawed at least twice and still give rise to larvae that undergo metamorphosis.
conclusionsThe simple methods we describe work well for both species, achieving >90% embryo development and producing larvae that undergo metamorphosis to juvenile adults. We hope that these methods will be useful to others working on marine invertebrate sperm.
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