Evidence map›Paper›PMID 38339058›Full record

ArticleInternational journal of molecular sciences2024

The Potential of PIP3 in Enhancing Wound Healing.

Yossi Blitsman, Etili Hollander, Chen Benafsha, Ksenia M Yegodayev, Uzi Hadad, Riki Goldbart, Tamar Traitel, Assaf Rudich, Moshe Elkabets, Joseph Kost

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Spatiotemporal distribution of PTEN before directed cell migration in monolayers.In vitro cellular & developmental biology. Animal · 2024
    Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yossi BlitsmanDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Etili HollanderDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Chen BenafshaDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Ksenia M YegodayevThe Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.ORCID 0000-0003-0721-838X
Uzi HadadThe Ilse Katz Institute for Nanoscale Science and Technology, Marcus Campus, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Riki GoldbartDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Tamar TraitelDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
Assaf RudichDepartment of Clinical Biochemistry and Pharmacology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.ORCID 0000-0002-1366-1444
Moshe ElkabetsThe Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.ORCID 0000-0003-3634-9098
Joseph KostDepartment of Chemical Engineering, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.ORCID 0000-0002-7854-533X
Ben-Gurion University of the Negev · IL

Funding

Israeli Ministry of Science and Technology through the Ze'ev Jabotinsky fellowship 3-16650Kreitman School of Advanced Graduate Studies of Ben-Gurion University through the High-tech, Bio-tech, and Chemo-tech fellowships 5809540600
6 · The paper itself

Abstract

Given the role of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in modulating cellular processes such as proliferation, survival, and migration, we hypothesized its potential as a novel therapeutic agent for wound closure enhancement. In this study, PIP3 was examined in its free form or as a complex with cationic starch (Q-starch) as a carrier. The intracellular bioactivity and localization of free PIP3 and the Q-starch/PIP3 complexes were examined. Our results present the capability of Q-starch to form complexes with PIP3, facilitate its cellular membrane internalization, and activate intracellular paths leading to enhanced wound healing. Both free PIP3 and Q-starch/PIP3 complexes enhanced monolayer gap closure in scratch assays and induced amplified collagen production within HaCAT and BJ fibroblast cells. Western blot presented enhanced AKT activation by free or complexed PIP3 in BJ fibroblasts in which endogenous PIP3 production was pharmacologically inhibited. Furthermore, both free PIP3 and Q-starch/PIP3 complexes expedited wound closure in mice, after single or daily dermal injections into the wound margins. Free PIP3 and the Q-starch/PIP3 complexes inherently activated the AKT signaling pathway, which is responsible for crucial wound healing processes such as migration; this was also observed in wound assays in mice. PIP3 was identified as a promising molecule for enhancing wound healing, and its ability to circumvent PI3K inhibition suggests possible implications for chronic wound healing.

Indexed as

Proto-Oncogene Proteins c-aktWound HealingAnimalsCell ProliferationFibroblastsMiceSignal TransductionStarchProto-Oncogene Proteins c-aktStarchcationic starch (Q-starch)drug deliveryintracellular deliveryphosphatidylinositol 3,4,5-trisphosphate (PIP3)wound healing

Identifiers

PMID38339058
PMCPMC10855400
OpenAlexW4391450006

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.