Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions.

Katarzyna Czerczak-Kwiatkowska, Marta Kaminska, Justyna Fraczyk, Ireneusz Majsterek, Beata Kolesinska

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Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Katarzyna Czerczak-KwiatkowskaFaculty of Chemistry, Institute of Organic Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

Marta KaminskaDivision of Biophysics, Institute of Materials Science and Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-924 Lodz, Poland.ORCID 0000-0002-3427-0944

Justyna FraczykFaculty of Chemistry, Institute of Organic Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.

Ireneusz MajsterekDepartment of Clinical Chemistry and Biochemistry, Medical University of Lodz, Narutowicza 60, 90-136 Lodz, Poland.

Beata KolesinskaFaculty of Chemistry, Institute of Organic Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.ORCID 0000-0002-4581-947X

Funding

National Science Center grant number UMO- 2018/31/B/ST8/02760

6 · The paper itself

Abstract

The aims of this study were to determine whether it is possible to use peptide microarrays obtained using the SPOT technique (immobilized on cellulose) and specific polyclonal antibodies to select fragments that reconstruct the outer sphere of proteins and to ascertain whether the selected peptide fragments can be useful in the study of their protein-protein and/or peptide-protein interactions. Using this approach, epidermal growth factor (EGF) fragments responsible for the interaction with the EGF receptor were searched. A library of EGF fragments immobilized on cellulose was obtained using triazine condensing reagents. Experiments on the interactions with EGFR confirmed the high affinity of the selected peptide fragments. Biological tests on cells showed the lack of cytotoxicity of the EGF fragments. Selected EGF fragments can be used in various areas of medicine.

Indexed as

Epidermal Growth FactorPeptidesAntibodiesCelluloseErbB ReceptorsPeptide FragmentsAntibodiesCelluloseEpidermal Growth FactorErbB ReceptorsPeptide FragmentsPeptidesinteraction with the receptorpolyclonal antibodiesprotein fragment microarrayregenerative medicineSPOT synthesisthe outer sphere of the protein

Identifiers

PMID38338748

DOI10.3390/ijms25031470

PMCPMC10855902

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