Evidence map›Paper›PMID 38337789›Full record

ArticleDiagnostics (Basel, Switzerland)2024

Near-Infrared In Vivo Imaging of Claudin-1 Expression by Orthotopically Implanted Patient-Derived Colonic Adenoma Organoids.

Sangeeta Jaiswal, Fa Wang, Xiaoli Wu, Tse-Shao Chang, Ahmad Shirazi, Miki Lee, Michael K Dame, Jason R Spence, Thomas D Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 98% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sangeeta JaiswalDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0001-6095-1666
Fa WangDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
Xiaoli WuDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
Tse-Shao ChangDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-1787-8744
Ahmad ShiraziDivision of Integrative System and Design, University of Michigan, Ann Arbor, MI 48109, USA.
Miki LeeDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-2634-5341
Michael K DameDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
Jason R SpenceDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.
Thomas D WangDivision of Gastroenterology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0001-9182-9620
University of Michigan · US

Funding

XenograftP30CA046592 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Eric R. Fearon · 1988 to 2026
$178.2M
Niche support of human intestinal stem cellsU01DK103141 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SPENCE, JASON · 2014 to 2023
$3.9M
Microsystems imaging system for epithelial-derived cancer heterogeneityU01EB028235 · NIBIB · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI OLDHAM, KENN R, WANG, THOMAS D · 2019 to 2023
$2.9M
Early detection of colorectal cancer in the traditional and serrated pathwaysR01CA249851 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WANG, THOMAS D · 2020 to 2024
$1.7M
NCI NIH HHS P30 CA046592NCI NIH HHS R01 CA249851NIBIB NIH HHS U01 EB028235NIDDK NIH HHS U01 DK103141NIDDK NIH HHS U01DK103141NIH HHS U01 EB028235 and R01 CA249851
6 · The paper itself

Abstract

backgroundClaudin-1 becomes overexpressed during the transformation of normal colonic mucosa to colorectal cancer (CRC).

methodsPatient-derived organoids expressed clinically relevant target levels and genetic heterogeneity, and were established from human adenoma and normal colons. Colonoids were implanted orthotopically in the colon of immunocompromised mice. This pre-clinical model of CRC provides an intact microenvironment and representative vasculature. Colonoid growth was monitored using white light endoscopy. A peptide specific for claudin-1 was fluorescently labeled for intravenous administration. NIR fluorescence images were collected using endoscopy and endomicroscopy.

resultsNIR fluorescence images collected using wide-field endoscopy showed a significantly greater target-to-background (T/B) ratio for adenoma versus normal (1.89 ± 0.35 and 1.26 ± 0.06) colonoids at 1 h post-injection. These results were confirmed by optical sections collected using endomicroscopy. Optical sections were collected in vivo with sub-cellular resolution in vertical and horizontal planes. Greater claudin-1 expression by individual epithelial cells in adenomatous versus normal crypts was visualized. A human-specific cytokeratin stain ex vivo verified the presence of human tissues implanted adjacent to normal mouse colonic mucosa.

conclusionsIncreased claudin-1 expression was observed from adenoma versus normal colonoids in vivo using imaging with wide field endoscopy and endomicrosopy.

Indexed as

adenomacolonoidconfocalendoscopyimagingpeptide

Identifiers

PMID38337789
PMCPMC10854921
OpenAlexW4391254307

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.