Evidence map›Paper›PMID 38336896›Full record

ArticleScientific reports2024

Sperm-specific histone H1 in highly condensed sperm nucleus of Sargassum horneri.

Yu Takeuchi, Shinya Sato, Chikako Nagasato, Taizo Motomura, Shujiro Okuda, Masahiro Kasahara, Fumio Takahashi, Shinya Yoshikawa

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Yu TakeuchiFaculty of Marine Science and Technology, Fukui Prefectural University, 1-1 Gakuencho, Obama, Fukui, 917-0003, Japan.
Shinya SatoFaculty of Marine Science and Technology, Fukui Prefectural University, 1-1 Gakuencho, Obama, Fukui, 917-0003, Japan.
Chikako NagasatoField Science Center for Northern Biosphere, Muroran Marine Station, Hokkaido University, Muroran, 051-0013, Japan.
Taizo MotomuraField Science Center for Northern Biosphere, Muroran Marine Station, Hokkaido University, Muroran, 051-0013, Japan.
Shujiro OkudaGraduate School of Medical and Dental Science, Niigata University, 1-757 Asahimachi, Chuoku, Niigata, Niigata, 951-8501, Japan.
Masahiro KasaharaGraduate School of Life Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan.
Fumio TakahashiGraduate School of Life Sciences, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan.
Shinya YoshikawaFaculty of Marine Science and Technology, Fukui Prefectural University, 1-1 Gakuencho, Obama, Fukui, 917-0003, Japan. syoshika@fpu.ac.jp.
Fukui Prefectural University · JPRitsumeikan University · JPHokkaido University · JPMuroran Institute of Technology · JPNiigata University · JP

Funding

Grants-in-Aid for Scientific Research No. 221S0002Grants-in-Aid for Scientific Research No. 26440156
6 · The paper itself

Abstract

Spermatogenesis is one of the most dramatic changes in cell differentiation. Remarkable chromatin condensation of the nucleus is observed in animal, plant, and algal sperm. Sperm nuclear basic proteins (SNBPs), such as protamine and sperm-specific histone, are involved in chromatin condensation of the sperm nucleus. Among brown algae, sperm of the oogamous Fucales algae have a condensed nucleus. However, the existence of sperm-specific SNBPs in Fucales algae was unclear. Here, we identified linker histone (histone H1) proteins in the sperm and analyzed changes in their gene expression pattern during spermatogenesis in Sargassum horneri. A search of transcriptomic data for histone H1 genes in showed six histone H1 genes, which we named ShH1.1a, ShH1b, ShH1.2, ShH1.3, ShH1.4, and ShH1.5. Analysis of SNBPs using SDS-PAGE and LC-MS/MS showed that sperm nuclei contain histone ShH1.2, ShH1.3, and ShH1.4 in addition to core histones. Both ShH1.2 and ShH1.3 genes were expressed in the vegetative thallus and the male and female receptacles (the organs producing antheridium or oogonium). Meanwhile, the ShH1.4 gene was expressed in the male receptacle but not in the vegetative thallus and female receptacles. From these results, ShH1.4 may be a sperm-specific histone H1 of S. horneri.

Indexed as

HistonesSargassumAnimalsCell NucleusChromatinChromatography, LiquidMaleSemenSpermatozoaTandem Mass SpectrometryChromatinHistones

Identifiers

PMID38336896
PMCPMC10858212
OpenAlexW4391677338

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.