ArticleScientific reports2024
Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The trial behind it
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Who cites it
6 citing papers in PubMed, 5 citations in OpenAlex.
- A comprehensive review of Onchocercidae nematodes (Nematoda; Filarioidea) infecting wild ruminant ungulates of North and Central America.International journal for parasitology. Parasites and wildlife · 2026Review
- Nuclear, mitochondrial, andmSphere · 2026Article
- Recurrence or Reinfection with Onchocerca lupi in a Child in 2021 and 2024 in New Mexico amid Rising Canine Infections.The American journal of tropical medicine and hygiene · 2025Article
- Prevalence of Onchocerca lupi in shelter dogs from an endemic region of the Southwestern USA.Parasites & vectors · 2025Article
- An indirect ELISA for the detection of antibodies against Dirofilaria spp. in cats.Parasites & vectors · 2025Article
- Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis.Pathogens (Basel, Switzerland) · 2024Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 5 countries.
Funding
Abstract
Onchocerca lupi is a zoonotic filarioid parasite of dogs and cats with widespread distribution. A specific non-invasive diagnostic assay for the detection of O. lupi infections remains unavailable. This study aimed to assess the accuracy, specificity, and sensitivity of an ELISA test designed using nine peptides from two O. lupi proteins. Sera (n = 54) collected from O. lupi infected dogs from endemic areas (Portugal and USA), alongside sera from dogs positive for Dirofilaria immitis, D. repens, Cercopithifilaria bainae, and Acanthocheilonema reconditum (n = 53) from a non-endemic area for O. lupi, as well as from helminth-free dogs (n = 60), were tested. The checkerboard titration method was applied for the optimization of peptide concentrations and conjugate anti-dog dilutions. Sensitivity, specificity, and optimal cut-off values were calculated using ROC curve analysis. All peptides reacted against sera of O. lupi, with no correlation between optic density (OD) values and microfilariae (mfs) loads. Sensitivity and specificity values ranging from 85.45 to 100%, and 88.89% to 100%, respectively, were recorded for all peptides examined, with 100% specificity and sensitivity observed for peptides 40_3, 40_5, 130_3, 120_3 and 40_1, 130_5, respectively. The maximum cut-off value was observed for peptides 40_5 (0.765) and 40_3 (0.708). Testing of sera from dogs positive for other filarioids resulted in lower OD values (up to 1.565) for peptides 40_3 and 40_5 when compared with O. lupi (up to 2.929). The availability of this assay will be of value in epidemiological studies of canine O. lupi infection in both endemic and non-endemic areas, and in assessing the risk for zoonotic transmission.
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Registered trials
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