Evidence map›Paper›PMID 38333191›Full record

ArticleRSC chemical biology2024

Evaluating Riboglow-FLIM probes for RNA sensing.

Nadia Sarfraz, Luke K Shafik, Zachary R Stickelman, Uma Shankar, Emilia Moscoso, Esther Braselmann

Open access · goldAbstract read
In one paragraph

Article in RSC chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Nadia SarfrazDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.ORCID https://orcid.org/0000-0002-6240-3898
Luke K ShafikDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.ORCID https://orcid.org/0009-0003-6777-1117
Zachary R StickelmanDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.ORCID https://orcid.org/0000-0002-3131-2993
Uma ShankarDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.
Emilia MoscosoDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.ORCID https://orcid.org/0000-0003-1688-3912
Esther BraselmannDepartment of Chemistry, Georgetown University Washington District of Columbia USA esther.braselmann@georgetown.edu.ORCID https://orcid.org/0000-0002-6877-1333
Georgetown University · US

Funding

Tissue Culture Shared ResourceP30CA051008 · NCI · GEORGETOWN UNIVERSITY · PI Louis M Weiner · 1990 to 2026
$71.5M
Illuminating multiplexed RNA dynamics to interrogate splicing in health and diseaseR35GM150823 · NIGMS · GEORGETOWN UNIVERSITY · PI Esther Braselmann · 2023 to 2026
$1.7M
RNA tools for probing spliceosome dynamicsR00GM127752 · NIGMS · GEORGETOWN UNIVERSITY · PI BRASELMANN, ESTHER · 2021 to 2023
$735k
NCI NIH HHS P30 CA051008NIGMS NIH HHS R00 GM127752NIGMS NIH HHS R35 GM150823
6 · The paper itself

Abstract

We recently developed Riboglow-FLIM, where we genetically tag and track RNA molecules in live cells through measuring the fluorescence lifetime of a small molecule probe that binds the RNA tag. Here, we systematically and quantitatively evaluated key elements of Riboglow-FLIM that may serve as the foundation for Riboglow-FLIM applications and further tool development efforts. Our investigation focused on measuring changes in fluorescence lifetime of representative Riboglow-FLIM probes with different linkers and fluorophores in different environments.

Identifiers

PMID38333191
PMCPMC10849122
OpenAlexW4390658664

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.