Evidence map›Paper›PMID 38329878›Full record

ArticleSTAR protocols2024

Protocol for measuring mechanical properties of live cells using atomic force microscopy.

Surya Bansi Singh, Shatruhan Singh Rajput, Shivprasad Patil, Deepa Subramanyam

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Surya Bansi SinghNational Centre for Cell Science, SP Pune University Campus, Pune 411007, India; SP Pune University, Pune 411007, India.
Shatruhan Singh RajputIndian Institute of Science Education and Research, Pune 411008, India; Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, CB2 1GA Cambridge, UK.
Shivprasad PatilIndian Institute of Science Education and Research, Pune 411008, India. Electronic address: s.patil@iiserpune.ac.in.
Deepa SubramanyamNational Centre for Cell Science, SP Pune University Campus, Pune 411007, India. Electronic address: deepa@nccs.res.in.
Indian Institute of Science Education and Research Pune · INNational Centre for Cell Science · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Atomic force microscope (AFM) is a powerful and versatile tool to determine the physical properties of cells. The force-distance curves obtained from AFM experiments can be used to determine the stiffness and viscoelastic properties of cells. Here, we present a protocol for the determination of viscoelasticity from live cells such as Drosophila hemocytes or mouse embryonic stem cells using AFM. This protocol has potential application in determining the physical properties of cells in healthy and diseased conditions. For complete details on the use and execution of this protocol, please refer to Mote et al. (2020),

Indexed as

Mechanical PhenomenaAnimalsMiceMicroscopy, Atomic ForceAFMAtomic Force MicroscopyBiophysicsCell BiologyModel OrganismsPhysics

Identifiers

PMID38329878
PMCPMC10865473
OpenAlexW4391629155

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.