Evidence map›Paper›PMID 38329653›Full record

ArticleMolecular biology reports2024

Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.

Kevin Maafu Juma, Kenta Morimoto, Vishnu Sharma, Kirti Sharma, Radhika Biyani, Manish Biyani, Teisuke Takita, Kiyoshi Yasukawa

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In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Kevin Maafu JumaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan.
Kenta MorimotoDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan.
Vishnu SharmaBioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan.
Kirti SharmaBioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan.
Radhika BiyaniBioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan.
Manish BiyaniBioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Lab, Ashahidai 2-13, Nomi City, Ishikawa, 923-1211, Japan. biyani@jaist.ac.jp.ORCID https://orcid.org/0000-0001-8761-7107
Teisuke TakitaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan.
Kiyoshi YasukawaDivision of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Sakyo-Ku, Kyoto, 606-8502, Japan. yasukawa.kiyoshi.7v@kyoto-u.ac.jp.ORCID http://orcid.org/0000-0001-7748-6043
Japan Advanced Institute of Science and Technology · JPKyoto University · JP

Funding

Japan Agency for Medical Research and Development 20he0622020h0001Japan Agency for Medical Research and Development 23hk0302011h0203Japan Science and Technology Agency JPMJTR20UUJapan Society for the Promotion of Science 18K19839Japan Society for the Promotion of Science 18KK0285Japan Society for the Promotion of Science 22H03332Japan Society for the Promotion of Science 23KJ1201
6 · The paper itself

Abstract

backgroundThe accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on transmission, virulence, and vaccine efficacy, and formulating public health interventions. In this study, a detection system utilizing micro temperature gradient gel electrophoresis (μTGGE) was developed for the identification of the D614 and G614 variants of the SARS-CoV-2 spike protein.

methodsThe in vitro synthesized D614 and G614 gene fragments of the SARS-CoV-2 spike protein were amplified via polymerase chain reaction and subjected to μTGGE analysis.

resultsThe migration patterns exhibited by the D614 and G614 variants on the polyacrylamide gel were distinctly dissimilar and readily discernible by μTGGE. In particular, the mid-melting pattern of D614 was shorter than that of G614.

conclusionsOur results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.

Indexed as

SARS-CoV-2Spike Glycoprotein, CoronavirusDenaturing Gradient Gel ElectrophoresisMutationSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2D614G mutationMelting patternMelting temperatureMicro temperature gradient gel electrophoresisMutation detectionSARS-CoV-2 spike protein

Identifiers

PMID38329653
OpenAlexW4391654495

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.