Evidence map›Paper›PMID 38328191›Full record

ArticlebioRxiv : the preprint server for biology2024

Multi-Channel smFRET study reveals a Compact conformation of EF-G on the Ribosome.

Jordan L Johnson, Jacob H Steele, Ran Lin, Victor G Stepanov, Miriam N Gavriliuc, Yuhong Wang

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jordan L JohnsonDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Jacob H SteeleDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Ran LinDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Victor G StepanovDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Miriam N GavriliucDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.
Yuhong WangDepartment of Biology and Biochemistry, University of Houston, Houston, TX 77204, USA.ORCID 0000-0002-1823-0272
University of Houston · US

Funding

HOUSTON AREA MOLECULAR BIOPHYSICS TRAINING PROGRAMT32GM008280 · NIGMS · RICE UNIVERSITY · PI WENSEL, THEODORE G · 1988 to 2023
$8.0M
EF-Tu mutagenesis and functional studyR01GM111452 · NIGMS · UNIVERSITY OF HOUSTON · PI YUHONG WANG, Shoujun Xu · 2015 to 2026
$3.2M
NIGMS NIH HHS R01 GM111452NIGMS NIH HHS T32 GM008280
6 · The paper itself

Abstract

While elongation factor G (EF-G) is crucial for ribosome translocation, the role of its GTP hydrolysis remains ambiguous. EF-G's indispensability is further exemplified by the phosphorylation of human eukaryotic elongation factor 2 (eEF2) at Thr56, which inhibits protein synthesis globally, but its exact mechanism is not clear. In this study, we developed a multi-channel single-molecule FRET (smFRET) microscopy methodology to examine the conformational changes of

Indexed as

compact EF-Ginduced-fitmulti-channel smFRETribosome translocationsingle molecule FRET

Identifiers

PMID38328191
PMCPMC10849647
OpenAlexW4391296485

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.