Evidence map›Paper›PMID 38328131›Full record

ArticlebioRxiv : the preprint server for biology2024

Quantification of local matrix deposition during muscle stem cell activation using engineered hydrogels.

Pamela Duran, Benjamin A Yang, Eleanor Plaster, Madeline Eiken, Claudia Loebel, Carlos A Aguilar

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Pamela DuranDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-8954-5722
Benjamin A YangDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0001-7412-6261
Eleanor PlasterDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0009-0005-2965-1485
Madeline EikenDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-2162-7422
Claudia LoebelDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0002-3140-5663
Carlos A AguilarDept. of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-3830-0634
University of Michigan · US

Funding

Research Education CoreP30AG024824 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lona Mody, RAYMOND L YUNG · 2004 to 2026
$29.2M
NIA NIH HHS P30 AG024824
6 · The paper itself

Abstract

Adult stem cells occupy a niche that contributes to their function, but how stem cells remodel their microenvironment remains an open-ended question. Herein, biomaterials-based systems and metabolic labeling were utilized to evaluate how skeletal muscle stem cells deposit extracellular matrix. Muscle stem cells and committed myoblasts were observed to generate less nascent matrix than muscle resident fibro-adipogenic progenitors. When cultured on substrates that matched the stiffness of physiological uninjured and injured muscles, the increased nascent matrix deposition was associated with stem cell activation. Reducing the ability to deposit nascent matrix in muscle stem cells attenuated function and mimicked impairments observed from muscle stem cells isolated from old aged muscles, which could be rescued with therapeutic supplementation of insulin-like growth factors. These results highlight how nascent matrix production is critical for maintaining healthy stem cell function.

Indexed as

biomaterialsextracellular matrixhyaluronic acidProtein labelingskeletal muscle

Identifiers

PMID38328131
PMCPMC10849481
OpenAlexW4391151365

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.