ArticleBiodiversity data journal2023
Enhancing DNA barcode reference libraries by harvesting terrestrial arthropods at the Smithsonian's National Museum of Natural History.
Article in Biodiversity data journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Prospects for Complete DNA Barcode Coverage of the New Zealand Insect Fauna.Journal of the Royal Society of New Zealand · 2026Article
- A Regional DNA Barcode Library for Northern Rocky Mountain Arthropods to Support Biodiversity and Molecular Ecological Research.Ecology and evolution · 2026Article
- DNA barcode reference library of bush-crickets (Orthoptera, Tettigoniidae) from the Iberian Peninsula.Scientific reports · 2025Article
- "Dark taxonomy": A new protocol for overcoming the taxonomic impediments for dark taxa and broadening the taxon base for biodiversity assessment.Cladistics : the international journal of the Willi Hennig Society · 2025Article
- Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library building methods.Scientific reports · 2025Article
- Building a reliable 16S mini-barcode library of wild bees from Occitania, south-west of France.Biodiversity data journal · 2025Article
- Taxonomy and Biological Control: New Challenges in an Old Relationship.Neotropical entomology · 2023Article
- A workflow for expanding DNA barcode reference libraries through 'museum harvesting' of natural history collections.Biodiversity data journal · 2023Article
- Developing the Protocol Infrastructure for DNA Sequencing Natural History Collections.Biodiversity data journal · 2023Article
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Authors and funding
27 authors.
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Abstract
The use of DNA barcoding has revolutionised biodiversity science, but its application depends on the existence of comprehensive and reliable reference libraries. For many poorly known taxa, such reference sequences are missing even at higher-level taxonomic scales. We harvested the collections of the Smithsonian's National Museum of Natural History (USNM) to generate DNA barcoding sequences for genera of terrestrial arthropods previously not recorded in one or more major public sequence databases. Our workflow used a mix of Sanger and Next-Generation Sequencing (NGS) approaches to maximise sequence recovery while ensuring affordable cost. In total, COI sequences were obtained for 5,686 specimens belonging to 3,737 determined species in 3,886 genera and 205 families distributed in 137 countries. Success rates varied widely according to collection data and focal taxon. NGS helped recover sequences of specimens that failed a previous run of Sanger sequencing. Success rates and the optimal balance between Sanger and NGS are the most important drivers to maximise output and minimise cost in future projects. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, the Global Genome Biodiversity Network Data Portal and the NMNH data portal.
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