Evidence map›Paper›PMID 38326837›Full record

ArticleCell communication and signaling : CCS2024

The scaffold of neutrophil extracellular traps promotes CCA progression and modulates angiogenesis via ITGAV/NFκB.

Congyi Zhang, Dehai Wu, Bowen Dong, Guanqun Liao, Yang Yu, Shizhuan Huang, Feng Luo, Bin Zhang, Haotian Wu, Tianwei Li and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication and signaling : CCS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
12.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Congyi Zhang *Department of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Dehai Wu *Department of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Bowen DongKey Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, The First Hospital of Jilin University, Changchun, Jilin, China.
Guanqun LiaoDepartment of Hepatobiliary Surgery, Foshan Hospital Affiliated to Southern Medical University, Foshan, China.
Yang YuDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Shizhuan HuangDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Feng LuoDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Bin ZhangDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Haotian WuDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Tianwei LiDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Dixiang WenDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Sheng TaiDepartment of hepatic surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, China. taisheng1973@163.com.
Harbin Medical University · CNSecond Affiliated Hospital of Harbin Medical University · CNJilin University · CNSouthern Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neutrophil extracellular traps (NETs) have garnered attention for their dual role in host defense and tumor promotion. With their involvement documented across a spectrum of tumors, their influence on the progression of cholangiocarcinoma (CCA) is of paramount interest. We employed immunohistochemistry and immunofluorescence to detect NET deposition in CCA tissues. Through in vitro and in vivo investigation, including CCA organoid and transposon-based models in PAD4 KO mice, we explored the effects of NETs on cell proliferation and metastasis. Molecular insights were gained through RNA sequencing, enzyme linked immunosorbent assay, and chromatin immunoprecipitation. Elevated intratumoral NET deposition within CCA tissues was associated with poor survival. The influence of NETs on CCA proliferation, migration and invasion was primarily mediated by NET-DNA. RNA sequencing unveiled the activation of the NFκB signaling pathway due to NET-DNA stimulation. NET-DNA pull-down assay coupled with mass spectrometry revealed the interaction between NET-DNA and αV integrin (ITGAV), culmination in the activation of the NFκB pathway. Furthermore, NET-DNA directly upregulated the expression of VEGF-A in cancer cells. The study unequivocally establishes NETs as facilitators of CCA progression, orchestrating proliferation, metastasis, and angiogenesis through ITGAV/NFκB pathway activation. This novel insight positions NETs as prospective therapeutic targets for managing CCA patients. By implementing a variety of methodologies and drawing intricate connections between NETs, DNA interactions, and signaling pathways, this research expands our comprehension of the complex interplay between the immune system and cancer progression, offering promising avenues for intervention.

Indexed as

Bile Duct NeoplasmsExtracellular TrapsAngiogenesisAnimalsBile Ducts, IntrahepaticDNAHumansMiceNeutrophilsDNAAngiogenesisCholangiocarcinomaIntegrin αVNeutrophil extracellular traps

Identifiers

PMID38326837
PMCPMC10851487
OpenAlexW4392732709

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.