Evidence map›Paper›PMID 38326288›Full record

ArticleJournal of extracellular vesicles2024

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.

Joshua A Welsh, Deborah C I Goberdhan, Lorraine O'Driscoll, Edit I Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A P Driedonks, Uta Erdbrügger and 63 more

Erratum issued 2 registry-linked trialsAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to 2 registered trials, which are not on this map. Cited by 2,991 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2,991citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07703969 phase1not yet recruitingnot on this mapstarted 2026, after this paper: background citation

A Phase 1 Split-Site Pilot Study to Evaluate the Safety and Dermal Tolerability of Topical Exosome-Containing Liquid in Healthy Adult Volunteers

TypeinterventionalSponsorWest Kazakhstan Marat Ospanov Medical UniversityRan2026 to 2027Enrolled10ConditionsSkin Irritation, Healthy VolunteersArmsExosome-Containing Liquid, Vehicle Liquid
NCT07707986 phase1not yet recruitingnot on this mapstarted 2026, after this paper: background citation

A Phase 1 Split-Site Pilot Study to Evaluate the Safety and Dermal Tolerability of Topical Exosome-Containing Liquid in Healthy Adult Volunteers Before Future Testing in Post-Melanoma-Excision Surgical Wounds

TypeinterventionalSponsorWest Kazakhstan Marat Ospanov Medical UniversityRan2026 to 2027Enrolled10ConditionsHealthy Volunteers (HV), Skin IrritationArmsExosome-Containing Liquid, Vehicle Liquid
3 · Its place in the literature

Who cites it

2,991 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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2,931 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

73 authors.

Joshua A WelshTranslational Nanobiology Section, Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.ORCID 0000-0002-1097-9756
Deborah C I GoberdhanNuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, UK.ORCID 0000-0003-0645-6714
Lorraine O'DriscollSchool of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland.
Edit I BuzasDepartment of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.ORCID 0000-0002-3744-206X
Cherie BlenkironFaculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Benedetta BussolatiDepartment of Molecular Biotechnology and Health Sciences, University of Turin, Turin, Italy.ORCID 0000-0002-3663-5134
Houjian CaiUniversity of Georgia, Athens, Georgia, USA.ORCID 0000-0003-4887-2652
Dolores Di VizioDepartment of Surgery, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Tom A P DriedonksDepartment CDL Research, University Medical Center Utrecht, Utrecht, The Netherlands.
Uta ErdbrüggerUniversity of Virginia Health System, Charlottesville, Virginia, USA.
Juan M Falcon-PerezExosomes Laboratory, Center for Cooperative Research in Biosciences, Basque Research and Technology Alliance, Derio, Spain.ORCID 0000-0003-3133-0670
Qing-Ling FuOtorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Andrew F HillInstitute for Health and Sport, Victoria University, Melbourne, Australia.ORCID 0000-0001-5581-2354
Metka LenassiFaculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.ORCID 0000-0002-9488-6855
Sai Kiang LimInstitute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Mỹ G MahoneyThomas Jefferson University, Philadelphia, Pennsylvania, USA.
Sujata MohantyStem Cell Facility, All India Institute of Medical Sciences, New Delhi, India.ORCID 0000-0002-0047-4914
Andreas MöllerChinese University of Hong Kong, Hong Kong, Hong Kong S.A.R.
Rienk NieuwlandLaboratory of Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.ORCID 0000-0002-5671-3400
Takahiro OchiyaTokyo Medical University, Tokyo, Japan.ORCID 0000-0002-0776-9918
Susmita SahooIcahn School of Medicine at Mount Sinai, New York, New York, USA.ORCID 0000-0002-7279-1564
Ana C TorrecilhasLaboratório de Imunologia Celular e Bioquímica de Fungos e Protozoários, Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo (UNIFESP) Campus Diadema, Diadema, Brazil.ORCID 0000-0001-5724-2199
Lei ZhengDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.ORCID 0000-0003-2576-8780
Andries ZijlstraDepartment of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.ORCID 0000-0001-8460-8803
Sarah AbuelreichDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Reem BagabasDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Paolo BergeseDepartment of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Esther M BridgesWeatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Marco BrucaleConsiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy.ORCID 0000-0001-7244-4389
Dylan BurgerKidney Research Centre, Ottawa Hopsital Research Institute, Ottawa, Canada.
Randy P CarneyDepartment of Biomedical Engineering, University of California, Davis, California, USA.ORCID 0000-0001-8193-1664
Emanuele CocucciDivision of Pharmaceutics and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio, USA.
Rossella CrescitelliSahlgrenska Center for Cancer Research, Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.ORCID 0000-0002-1714-3169
Edveena HanserDepartment of Biomedicine, University Hospital Basel, Basel, Switzerland.
Adrian L HarrisDepartment of Oncology, University of Oxford, Oxford, UK.
Norman J HaugheyDepartments of Neurology and Psychiatry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0001-5194-4122
An HendrixLaboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.
Alexander R IvanovBarnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.
Tijana Jovanovic-TalismanDepartment of Cancer Biology and Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Nicole A Kruh-GarciaBio-pharmaceutical Manufacturing and Academic Resource Center (BioMARC), Infectious Disease Research Center, Colorado State University, Fort Collins, Colorado, USA.
Vroniqa Ku'ulei-Lyn FaustinoDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Diego KyburzDepartment of Biomedicine, University of Basel, Basel, Switzerland.
Cecilia LässerKrefting Research Centre, Department of Internal Medicine and Clinical Nutrition, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Kathleen M LennonDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Jan LötvallKrefting Research Centre, Institute of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Adam L MaddoxDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Elena S Martens-UzunovaErasmus MC Cancer Institute, University Medical Center Rotterdam, Department of Urology, Rotterdam, The Netherlands.ORCID 0000-0002-5363-2525
Rachel R MizenkoDepartment of Biomedical Engineering, University of California, Davis, California, USA.
Lauren A NewmanCollege of Medicine and Public Health, Flinders University, Adelaide, Australia.ORCID 0000-0003-3303-1666
Andrea RidolfiDepartment of Physics and Astronomy, and LaserLaB Amsterdam, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Eva RohdeDepartment of Transfusion Medicine, University Hospital, Salzburger Landeskliniken GmbH of Paracelsus Medical University, Salzburg, Austria.ORCID 0000-0001-8692-886X
Tatu RojalinDepartment of Biomedical Engineering, University of California, Davis, California, USA.
Andrew RowlandCollege of Medicine and Public Health, Flinders University, Adelaide, Australia.
Andras SafticsDepartment of Molecular Medicine, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, California, USA.
Ursula S SandauDepartment of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA.ORCID 0000-0002-3646-7089
Julie A SaugstadDepartment of Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA.ORCID 0000-0002-2996-9611
Faezeh ShekariDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.ORCID 0000-0001-6026-5412
Simon SwiftWaipapa Taumata Rau University of Auckland, Auckland, New Zealand.
Dmitry Ter-OvanesyanWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
Juan P TosarUniversidad de la República, Montevideo, Uruguay.ORCID 0000-0002-2021-2479
Zivile UseckaiteCollege of Medicine and Public Health, Flinders University, Adelaide, Australia.
Francesco ValleConsiglio Nazionale delle Ricerche - Istituto per lo Studio dei Materiali Nanostrutturati, Bologna, Italy.
Zoltan VargaBiological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, Budapest, Hungary.
Edwin van der PolAmsterdam Vesicle Center, Amsterdam University Medical Centers, Location AMC, University of Amsterdam, Amsterdam, The Netherlands.
Martijn J C van HerwijnenDepartment of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Marca H M WaubenDepartment of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.ORCID 0000-0003-0360-0311
Ann M WehmanUniversity of Denver, Denver, USA.ORCID 0000-0001-9826-4132
Sarah WilliamsInternational Society for Extracellular Vesicles.
Andrea ZendriniDepartment of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.
Alan J ZimmermanBarnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, Northeastern University, Boston, Massachusetts, USA.ORCID 0000-0001-6280-4790
MISEV Consortium
Clotilde ThéryInstitut Curie, INSERM U932, PSL University, Paris, France.ORCID 0000-0001-8294-6884
Kenneth W WitwerDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.ORCID 0000-0003-1664-4233

Funding

Vironomics and Biostatistics CoreP01CA019014 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI BLOSSOM A DAMANIA, NANCY JOAN RAAB-TRAUB · 1985 to 2026
$43.8M
Translational Analysis CoreP30DK058404 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI MARY Kay WASHINGTON · 2002 to 2026
$29.9M
The role of prestimulus oscillatory brain dynamics in auditory memoryP20GM113109 · NIGMS · KANSAS STATE UNIVERSITY · PI David Edward Thompson · 2017 to 2026
$24.1M
PRINCIPLES IN PULMONARY RESEARCHT32HL007317 · NHLBI · WASHINGTON UNIVERSITY · PI Steven Brody, Michael J Holtzman · 1985 to 2026
$16.1M
Roles for Supermeres in CRC ProgressionP01CA229123 · NCI · VANDERBILT UNIVERSITY · PI Alissa M Weaver · 2020 to 2026
$12.9M
Training/Dissemination-Resource for Molecular Imaging Agents in Precision MedicineP41EB024495 · NIBIB · UT SOUTHWESTERN MEDICAL CENTER · PI MARTIN G POMPER · 2017 to 2026
$11.8M
Training Grant in Molecular Imaging Research (MGH/HMS)T32CA079443 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI WEISSLEDER, MD, PHD, RALPH · 2000 to 2025
$10.1M
Functional role and therapeutic targeting of exosomes and extracellular RNA biomarkers in heart failureR35HL150807 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI Saumya Das · 2020 to 2026
$6.7M
A Clinical Indications Prediction (CLIP) Scale for Human Mesenchymal Stem CellsR01HL144089 · NHLBI · UNIVERSITY OF FLORIDA · PI Donald G Phinney · 2018 to 2026
$5.4M
Exosome secretion in breast cancer progressionR01CA206458 · NCI · VANDERBILT UNIVERSITY · PI PONIK, SUZANNE MARIE, WEAVER, ALISSA M · 2016 to 2025
$4.6M
Roles of Recycling Endosomes in Neuronal Extracellular Vesicle Cargo TrafficR01NS103967 · NINDS · BRANDEIS UNIVERSITY · PI Avital Adah Rodal · 2018 to 2026
$4.5M
Next-generation extracellular vesicle biologics to target central nervous system and peripheral reservoirs of HIVR01AI144997 · NIAID · JOHNS HOPKINS UNIVERSITY · PI RAMRATNAM, BHARAT, WITWER, KENNETH W · 2019 to 2023
$4.0M
British Heart Foundation FS/IBSRF/22/25110Medical Research Council MR/R025096/1Medical Research Council MR/S009426/1NCATS NIH HHS U18 TR003807NCATS NIH HHS UH3 TR002881NCATS NIH HHS UH3 TR002884NCATS NIH HHS UH3 TR002886NCI NIH HHS K22 CA282019NCI NIH HHS K99 CA277242NCI NIH HHS P01 CA019014NCI NIH HHS P01 CA229123NCI NIH HHS R00 CA277242NCI NIH HHS R01 CA206458NCI NIH HHS R01 CA218500NCI NIH HHS R01 CA249424NCI NIH HHS R01 CA249684NCI NIH HHS R01 CA270251NCI NIH HHS T32 CA079443NCI NIH HHS U01 CA224276NCI NIH HHS UH3 CA241694NEI NIH HHS R01 EY034193NEI NIH HHS R21 EY033057NEI NIH HHS R21 EY035425NEI NIH HHS R56 EY034193NHLBI NIH HHS DP2 HL152426NHLBI NIH HHS R01 HL144089NHLBI NIH HHS R35 HL150807NHLBI NIH HHS T32 HL007317NIAID NIH HHS R01 AI144997NIAID NIH HHS R01 AI158749NIAID NIH HHS R01 AI168142NIAID NIH HHS R01 AI174273NIAMS NIH HHS R01 AR072735NIA NIH HHS R01 AG081807NIA NIH HHS RF1 AG059392NIBIB NIH HHS K99 EB033857NIBIB NIH HHS P41 EB024495NIDA NIH HHS R01 DA047807NIDCR NIH HHS L30 DE029070NIDDK NIH HHS P30 DK058404NIDDK NIH HHS R01 DK136511NIGMS NIH HHS P20 GM113109NIGMS NIH HHS R01 GM138778NIGMS NIH HHS R01 GM148708NIGMS NIH HHS R35 GM136421NIGMS NIH HHS R35 GM149203NIGMS NIH HHS R35 GM151259NIMH NIH HHS K99 MH126950NIMH NIH HHS L30 MH131119NIMH NIH HHS R01 MH134389NIMH NIH HHS R33 MH118164NINDS NIH HHS R01 NS103967
6 · The paper itself

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Indexed as

ExosomesExtracellular VesiclesBiological TransportBiomarkersPhenotypeBiomarkersectosomesexosomesextracellular particlesextracellular vesiclesguidelinesmicroparticlesmicrovesiclesminimal information requirementsMISEVreproducibilityrigorstandardisation

Identifiers

PMID38326288
PMCPMC10850029

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.