Evidence map›Paper›PMID 38322536›Full record

ArticleSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition2024

[Piezo1 Mediates the Regulation of Substrate Stiffness on Primary Cilia in Chondrocytes].

Huaqing Guo, Minhua Lan, Qiang Zhang, Yanli Liu, Yanjun Zhang, Quanyou Zhang, Weiyi Chen

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Huaqing Guo（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Minhua Lan（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Qiang Zhang（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Yanli Liu（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Yanjun Zhang（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Quanyou Zhang（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Weiyi Chen（ 030024） College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To investigate how substrate stiffness regulates the morphology of primary cilia in chondrocytes and to illustrate how Piezo1 mediates the morphology regulation of primary cilia by substrate stiffness. Methods: Polydimethylsiloxane (PDMS) curing agent and the main agent (Dow Corning, Beijing, China) were mixed at the ratio of 1∶10 (stiff), 1∶50 (medium stiffness), and 1∶70 (soft), respectively, to prepare substrate films with the thickness of 1 mm at different levels of stiffness, including stiff substrate of (2.21±0.12) MPa, medium-stiffness substrate of (54.47±6.06) kPa, and soft substrate of (2.13±0.10) kPa. Chondrocytes were cultured with the substrates of three different levels of stiffness. Then, the cells were treated with Tubastatin A (Tub A) to inhibit histone deacetylase 6 (HDAC6), Piezo1 activator Yoda1, and inhibitor GsMTx4, respectively. The effects of HDAC6, Yoda1, and GsMTx4 on chondrocyte morphology and the length of primary cilia were analyzed through immunofluorescence staining. Results: The stiff substrate increased the spread area of the chondrocytes. Immunofluorescence assays showed that the cytoskeleton and the nuclear area of the cells on the stiff substrate were significantly increased ( Conclusion: Both substrate stiffness and Piezo1 may affect the morphology of chondrocyte primary cilia by regulating HDAC6 activity.

Indexed as

ChondrocytesCiliaIon ChannelsCells, CulturedCytoskeletonIon ChannelsChondrocyteCytoskeletonHDAC6Piezo1Primary ciliaSubstrate stiffness

Identifiers

PMID38322536
PMCPMC10839480

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.