Evidence map›Paper›PMID 38322510›Full record

ArticleSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition2024

[Establishment and Evaluation of a Resazurin-Based Microdilution Assay for Microbial Sensitivity Test of

Menghuan Li, Guiqin Yang, Youwei Wang, Gang Yong, Hongren Wang, Mingjiang Bie, Guoqing Wang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. [A NovelSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Menghuan Li/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Guiqin Yang/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Youwei Wang/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Gang Yong/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Hongren Wang/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Mingjiang Bie/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Guoqing Wang/ （ 610041） West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To establish and evaluate a microbial sensitivity test method for Methods: Based on the broth microdilution method, resazurin was added as a live bacteria indicator. WHO G, a WHO gonococcal reference strain, was used to optimize the incubation time for resazurin-stained bacteria and the color change was visually observed to obtain the results. Agar dilution method (the gold standard) and resazurin-based microdilution assay were used to determine the minimum inhibitory concentration (MIC) of azithromycin, ceftriaxone, and spectinomycin for 3 reference strains and 32 isolates of Results: The results obtained 6 hours after resazurin was added were consistent with those of the agar dilution method and the resazurin-based microdilution assay was established accordingly based on this parameter. The EA of resazurin-based microdilution assay for measuring the MIC results of azithromycin, ceftriaxone, and spectinomycin was 97.1%, 91.5%, and 94.3%, respectively, and the CA was 88.6%, 94.3%, and 94.3%, respectively. The VME was 0% for all three antibiotics, while the ME was 11.4%, 5.7%, and 5.7%, respectively. Conclusion: The resazurin-based microdilution assay established in this study showed good agreement with agar dilution method for measuring the MIC of antibiotics against

Indexed as

AzithromycinNeisseria gonorrhoeaeOxazinesXanthenesAgarAnti-Bacterial AgentsCeftriaxoneDrug Resistance, BacterialMicrobial Sensitivity TestsSpectinomycinAgarAnti-Bacterial AgentsAzithromycinCeftriaxoneOxazinesresazurinSpectinomycinXanthenesMicrobial sensitivity testNeisseria gonorrhoeaeResazurin-based microdilution assay

Identifiers

PMID38322510
PMCPMC10839469

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.