ArticleNeurophotonics2024
Contribution of optical resolution to the spatial precision of two-photon optogenetic photostimulation
Article in Neurophotonics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 8 citations in OpenAlex.
- Neuroimmune circuits in respiratory pathophysiology: decoding molecular crosstalk for precision therapeutic targeting.Annals of medicine · 2026Review
- Computational optimization of two-photon holographic stimulation sitesJournal of neural engineering · 2026Article
- Desensitization of opsin responses during all-optical interrogation depends on imaging parameters.Neurophotonics · 2026Article
- A software platform for real-time and adaptive neuroscience experiments.Nature communications · 2025Article
- Network dynamics underlying activity-timescale differences between cortical regions.bioRxiv : the preprint server for biology · 2025Article
- Fast photostimulus optimization for holographic control of neural ensemble activitybioRxiv : the preprint server for biology · 2025Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Significance: Two-photon optogenetics combines nonlinear excitation with noninvasive activation of neurons to enable the manipulation of neural circuits with a high degree of spatial precision. Combined with two-photon population calcium imaging, these approaches comprise a flexible platform for all-optical interrogation of neural circuits. However, a multitude of optical and biological factors dictate the exact precision of this approach Aim: We aimed to assess how the optical point spread function (OPSF) contributes to the spatial precision of two-photon photostimulation in neurobiology. Approach: We altered the axial spread of the OPSF of the photostimulation beam using a spatial light modulator. Subsequently, calcium imaging was used to monitor the axial spatial precision of two-photon photostimulation of layer 2 neurons in the mouse neocortex. Results: We found that optical resolution is not always the limiting factor of the spatial precision of two-photon optogenetic photostimulation and, by doing so, reveal the key factors that must be improved to achieve maximal precision. Conclusions: Our results enable future work to focus on the optimal factors by providing key insight from controlled experiments in a manner not previously reported. This research can be applied to advance the state-of-the-art of all-optical interrogation, extending the toolkit for neuroscience research to achieve spatiotemporal precision at the crucial levels in which neural circuits operate.
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Registered trials
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