Evidence map›Paper›PMID 38319573›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Measuring Pericellular Oxygen Tension for In Vitro Cell Culture.

Zachary J Rogers, Khushbu Bhatt, Sidi A Bencherif

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Making sense of oxygen sensing.The EMBO journal · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Zachary J RogersDepartment of Chemical Engineering, Northeastern University, Boston, MA, USA.
Khushbu BhattDepartment of Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
Sidi A BencherifDepartment of Chemical Engineering, Northeastern University, Boston, MA, USA. s.bencherif@northeastern.edu.

Funding

Overcoming vaccine-associated hypoxia with advanced biomaterials to enhance cancer immunotherapyR01EB027705 · NIBIB · NORTHEASTERN UNIVERSITY · PI HATFIELD, STEPHEN MATTHEW · 2021 to 2024
$1.9M
NIBIB NIH HHS R01 EB027705
6 · The paper itself

Abstract

Cell culture, the process of growing cells in conditions that mimic those in the body, is a key technique in biomedical research. Oxygen is not controlled in conventional cell culture, although chambers that control oxygen in the surrounding gas phase are commercially available. In both cases, it is valuable to understand the pericellular oxygen tension (i.e., the oxygen concentration that cells experience) in cultures. Herein we describe a procedure for using commercial optical sensor spots to measure pericellular oxygen for adherent and suspension cultures. Spots are placed on surfaces on which cells are grown, and optical cables are attached to the outside of the cell culture vessels and connected to a computer. Associated software allows for the real-time monitoring of pericellular oxygen during cell culture experiments. This procedure enhances the reproducibility and control of cell culture.

Indexed as

Biomedical ResearchCell Culture TechniquesBlood Gas AnalysisOxygenReproducibility of ResultsOxygenCell cultureHypoxiaLuminescenceOptical sensorOxygenPericellular

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.