Evidence map›Paper›PMID 38319406›Full record

ArticlePlanta2024

Intragenic Agrobacterium-mediated gene transfer mimics micro-translocations without foreign DNA.

Philippa J Barrell, Julie M Latimer, Timothy R Millar, Jeanne M E Jacobs, Anthony J Conner

Abstract read
In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Philippa J BarrellNew Zealand Institute for Plant and Food Research Ltd, Private Bag 4704, Christchurch, New Zealand. philippa.barrell@plantandfood.co.nz.ORCID http://orcid.org/0000-0003-2633-7616
Julie M LatimerNew Zealand Institute for Plant and Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.ORCID http://orcid.org/0009-0003-0929-9002
Timothy R MillarNew Zealand Institute for Plant and Food Research Ltd, Private Bag 4704, Christchurch, New Zealand.ORCID http://orcid.org/0000-0002-5142-8811
Jeanne M E JacobsAgResearch Ltd, Private Bag 4749, Christchurch, New Zealand.ORCID http://orcid.org/0000-0002-1047-1039
Anthony J ConnerAgResearch Ltd, Private Bag 4749, Christchurch, New Zealand. tony.conner@agresearch.co.nz.ORCID http://orcid.org/0000-0003-4857-1761

Funding

New Zealand Foundation for Research, Science and Technology C02X0203
6 · The paper itself

Abstract

MAIN

conclusionAgrobacterium-mediated transformation of Nicotiana tabacum, using an intragenic T-DNA region derived entirely from the N. tabacum genome, results in the equivalence of micro-translocations within genomes. Intragenic Agrobacterium-mediated gene transfer was achieved in Nicotiana tabacum using a T-DNA composed entirely of N. tabacum DNA, including T-DNA borders and the acetohydroxyacid synthase gene conferring resistance to sulfonylurea herbicides. Genomic analysis of a resulting plant, with single locus inheritance of herbicide resistance, identified a single insertion of the intragenic T-DNA on chromosome 5. The insertion event was composed of three N. tabacum DNA fragments from other chromosomes, as assembled on the T-DNA vector. This validates that intragenic transformation of plants can mimic micro-translocations within genomes, with the absence of foreign DNA.

Indexed as

Acetolactate SynthaseGene RearrangementAgrobacteriumDNANicotianaTranslocation, GeneticAcetolactate SynthaseDNAAcetohydroxyacid synthaseHerbicide resistanceIntragenic vectorsMicro-translocationsNicotiana tabacum

Identifiers

PMID38319406
PMCPMC10847175

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.