ArticleNature methods2024
Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules.
Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Review
- Distinct 5' and 3' coverage biases shape transcriptome interpretation in Nanopore direct RNA versus PCR-cDNA sequencing.BMC genomics · 2026Article
- Umi-pipeline-nf: a modular and scalable workflow for UMI-tagged nanopore amplicon analysis with real-time sequencing integration and GPU-acceleration.Bioinformatics (Oxford, England) · 2026Article
- High-sensitivity, protein-independent detection of dsDNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reducing bias and enhancing equity in AI-enabled precision nutrition: addressing measurement error across wearables, multiomics, and dietary data.Frontiers in digital health · 2026Review
- Evaluation of false positive and false negative errors in targeted next generation sequencing.Genome biology · 2025Article
- Structure-enhanced deep learning accelerates aptamer selection for small molecule families like steroids.Briefings in bioinformatics · 2025Article
- Medium-sized protein language models perform well at transfer learning on realistic datasets.Scientific reports · 2025Article
- Medium-sized protein language models perform well at transfer learning on realistic datasets.bioRxiv : the preprint server for biology · 2025Article
- AsaruSim: a single-cell and spatial RNA-Seq Nanopore long-reads simulation workflow.Bioinformatics (Oxford, England) · 2025Article
- Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences.Communications biology · 2025Article
- Construction of edit-distance graphs for large sets of short reads through minimizer-bucketing.Bioinformatics advances · 2025Article
- Single-cell nuclear RNA-sequencing reveals dynamic changes in breast muscle cells during the embryonic development of Ding'an goose.PloS one · 2025Article
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- scTrends: A living review of commercial single-cell and spatial 'omic technologies.Cell genomics · 2024Review
- High-sensitive spatially resolved T cell receptor sequencing with SPTCR-seq.Nature communications · 2023Article
Corrections and comments
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Authors and funding
14 authors.
Funding
Abstract
Unique molecular identifiers are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating absolute counts of RNA molecules is underappreciated. We show that PCR errors are a source of inaccuracy in both bulk and single-cell sequencing data, and synthesizing unique molecular identifiers using homotrimeric nucleotide blocks provides an error-correcting solution that allows absolute counting of sequenced molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.