ArticleMetabolic engineering2024
A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways.
Article in Metabolic engineering, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Construction of a pathway-independent three-module self-induced regulatory system and its application in 3-hydroxypropionic acid production.Engineering microbiology · 2026Article
- Gold nanoparticle-mediated metabolic engineering in Trichoderma longibrachiatum MD33 unveils a hybrid terpenoid-alkaloid pathway for enhanced dendrobine biosynthesis.Microbial cell factories · 2026Article
- Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Characterizing and Engineering a Succinate-Responsive Biosensor System inACS synthetic biology · 2025Article
- Engineering genetic circuits for dynamic control of central metabolism.Metabolic engineering · 2025Article
- Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement.Scientific reports · 2025Article
- Engineering an Overflow-Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production.Biotechnology and bioengineering · 2025Article
- A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli.Microbial cell factories · 2025Article
- Architecting a transcriptional repressor-based genetic inverter for tryptophan derived pathway regulation in Escherichia coli.Metabolic engineering · 2024Article
- Investigating and Engineering an 1,2-Propanediol-Responsive Transcription Factor-Based Biosensor.ACS synthetic biology · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Microbial synthesis has emerged as a promising and sustainable alternative to traditional chemical synthesis and plant extraction. However, the competition between synthetic pathways and central metabolic pathways for cellular resources may impair final production efficiency. Moreover, when the synthesis of target product requires multiple precursors from the same node, the conflicts of carbon flux have further negative impacts on yields. In this study, a self-regulated network was developed to relieve the competition of precursors in complex synthetic pathways. Using 4-hydroxycoumarin (4-HC) synthetic pathway as a proof of concept, we employed an intermediate as a trigger to dynamically rewire the metabolic flux of pyruvate and control the expression levels of genes in 4-HC synthetic pathway, achieving self-regulation of multiple precursors and enhanced titer. Transcriptomic analysis results additionally demonstrated that the gene transcriptional levels of both pyruvate kinase PykF and synthetic pathway enzyme SdgA dynamically changed according to the intermediate concentrations. Overall, our work established a self-regulated network to dynamically balance the metabolic flux of two precursors in 4-HC biosynthesis, providing insight into balancing biosynthetic pathways where multiple precursors compete and interfere with each other.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.