Mammalian Antigen Display for Pandemic Countermeasures.

Andrea Quezada, Ankur Annapareddy, Kamyab Javanmardi, John Cooper, Ilya J Finkelstein

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andrea Quezada *Department of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA.

Ankur Annapareddy *Department of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA.

Kamyab JavanmardiDepartment of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA.

John CooperDepartment of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA.

Ilya J FinkelsteinDepartment of Molecular BioSciences, University of Texas at Austin, Austin, TX, USA. ilya@finkelsteinlab.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pandemic countermeasures require the rapid design of antigens for vaccines, profiling patient antibody responses, assessing antigen structure-function landscapes, and the surveillance of emerging viral lineages. Cell surface display of a viral antigen or its subdomains can facilitate these goals by coupling the phenotypes of protein variants to their DNA sequence. Screening surface-displayed proteins via flow cytometry also eliminates time-consuming protein purification steps. Prior approaches have primarily relied on yeast as a display chassis. However, yeast often cannot express large viral glycoproteins, requiring their truncation into subdomains. Here, we describe a method to design and express antigens on the surface of mammalian HEK293T cells. We discuss three use cases, including screening of stabilizing mutations, deep mutational scanning, and epitope mapping. The mammalian antigen display platform described herein will accelerate ongoing and future pandemic countermeasures.

Indexed as

PandemicsSaccharomyces cerevisiaeAnimalsAntibodies, ViralEpitope MappingEpitopesHEK293 CellsHemagglutinin Glycoproteins, Influenza VirusHumansMammalsAntibodies, ViralEpitopesHemagglutinin Glycoproteins, Influenza VirusAntigenCoronavirusFlow cytometryHemagglutininInfluenzaSpikeSurface display

Identifiers

PMID38315367

DOI10.1038/s41401-020-0485-4

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.