Evidence map›Paper›PMID 38315361›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain.

Catherine Forest-Nault, Izel Koyuturk, Jimmy Gaudreault, Alex Pelletier, Denis L'Abbé, Brian Cass, Louis Bisson, Alina Burlacu, Laurence Delafosse, Matthew Stuible and 3 more

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Catherine Forest-NaultDepartment of Chemical Engineering, Polytechnique Montreal, Montreal, QC, Canada.
Izel KoyuturkHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Jimmy GaudreaultDepartment of Chemical Engineering, Polytechnique Montreal, Montreal, QC, Canada.
Alex PelletierHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Denis L'AbbéHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Brian CassHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Louis BissonHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Alina BurlacuHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Laurence DelafosseHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Matthew StuibleHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada.
Olivier HenryDepartment of Chemical Engineering, Polytechnique Montreal, Montreal, QC, Canada.
Gregory De CrescenzoDepartment of Chemical Engineering, Polytechnique Montreal, Montreal, QC, Canada. gregory.decrescenzo@polymtl.ca.
Yves DurocherHuman Health Therapeutics Research Centre, National Research Council of Canada, Montreal, QC, Canada. yves.durocher@cnrc-nrc.gc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surface plasmon resonance (SPR)-based biosensing enables the characterization of protein-protein interactions. Several SPR-based approaches have been designed to evaluate the binding mechanism between the angiotensin-converting enzyme 2 (ACE2) receptor and the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein leading to a large range of kinetic and thermodynamic constants. This chapter describes a robust SPR assay based on the K5/E5 coiled-coil capture strategy that reduces artifacts. In this method, ACE2 receptors were produced with an E5-tag and immobilized as ligands in the SPR assay. This chapter details methods for high-yield production and purification of the studied proteins, functionalization of the sensor chip, conduction of the SPR assay, and data analysis.

Indexed as

Biosensing TechniquesCOVID-19Angiotensin-Converting Enzyme 2HumansProtein BindingSARS-CoV-2Spike Glycoprotein, CoronavirusAngiotensin-Converting Enzyme 2Spike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Angiotensin-converting enzyme 2 (ACE2) receptorKinetic modelLigand-oriented captureReceptor-binding domain (RBD)SARS-CoV-2, high yield protein productionSPR data analysisSurface plasmon resonance (SPR)

Identifiers

PMID38315361

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.