Evidence map›Paper›PMID 38315360›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

SARS-CoV-2S-Protein-Ace2 Binding Analysis Using Surface Plasmon Resonance.

Jason Baardsnes, Béatrice Paul-Roc

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Jason BaardsnesQuality Attributes and Characterization, Human Health Therapeutics, National Research Council Canada, Montréal, QC, Canada. Jason.Baardsnes@cnrc-nrc.gc.ca.
Béatrice Paul-RocQuality Attributes and Characterization, Human Health Therapeutics, National Research Council Canada, Montréal, QC, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surface plasmon resonance (SPR) allows for the label-free determination of the binding affinity and rate constants of bimolecular interactions. Here, we describe the method used for the analysis of the Ace2-SARS-CoV2 S-protein interaction using indirect capture of the S-protein onto the SPR surface, and flowing monomeric Ace2. This method will allow for the determination of the rate constants for affinity, with additional analysis that is achievable using S-protein capture levels in conjunction with the sensorgram response for relative activity benchmarking.

Indexed as

COVID-19Surface Plasmon ResonanceHumansProtein BindingRNA, ViralSARS-CoV-2Spike Glycoprotein, CoronavirusRNA, ViralSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2Ace2Affinity (KD)AssociationBindingBinding kineticsDissociationLabel-freeSARS CoV-2S-proteinSurface plasmon resonance (SPR)

Identifiers

PMID38315360

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.