ArticleParticle and fibre toxicology2024
The in vitro gastrointestinal digestion-associated protein corona of polystyrene nano- and microplastics increases their uptake by human THP-1-derived macrophages.
Article in Particle and fibre toxicology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Mapping Microplastics in Humans: Analysis of Polymer Types, and Shapes in Food and Drinking Water-A Systematic Review.International journal of molecular sciences · 2024Pooled it
- Fluorescent Labeling Strategies for Tracking Micro- and Nanoplastics in Biological Systems.Materials (Basel, Switzerland) · 2026Review
- Review
- Microplastics and Nanoplastics in Food: Dietary Toxicology Beyond Particle Counts.Comprehensive reviews in food science and food safety · 2026Review
- Microplastics, nanoplastics and liver disease: an emerging health concern?Nature reviews. Gastroenterology & hepatology · 2026Review
- Storage-Dependent Changes in Microplastic-Associated Recoverable Residues in Yogurt ContainingToxics · 2026Article
- Investigating the microwave degradation of polypropylene microplastics and their impact on human intestinal cell models.Toxicology reports · 2026Article
- Dynamic Uptake and Elimination of Microplastics byEnvironment & health (Washington, D.C.) · 2026Article
- Microplastics and Nanoplastics in the Human Intestine: A Review of Health Implications and Possible Dietary Interventions.Advances in nutrition (Bethesda, Md.) · 2026Review
- Review
- Lessons learned from allergen adsorption on engineered nanomaterials: bridging experimental insights and computational technologies.Nanomedicine (London, England) · 2026Review
- Review
- Microplastics and nanoplastics in human health: a comprehensive review of exposure pathways, cellular mechanisms, and toxicological implications.Archives of toxicology · 2026Review
- Osteoimmunological impacts of micro/nanoplastics: systemic translocation, inflammatory responses, and bone remodeling disruption.Frontiers in immunology · 2026Review
- Pathogenesis of Micro/Nanoplastics in Mammalian Systems: Gut to Systemic Multi-Organ Dysfunction.Research (Washington, D.C.) · 2026Review
- Review
- Polystyrene Nanoplastics in Human Gastrointestinal Models-Cellular and Molecular Mechanisms of Toxicity.International journal of molecular sciences · 2025Review
- Protein Corona Stability and Removal from PET Microplastics: Analytical and Spectroscopic Evaluation in Simulated Intestinal Conditions.Foods (Basel, Switzerland) · 2025Article
- Plastic induced urinary tract disease and dysfunction: a scoping review.Journal of exposure science & environmental epidemiology · 2025Article
- The interactions of non-functionalized polystyrene nanoparticles with human albumin and erythrocyte proteins: implications and potential consequences.Scientific reports · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMicro- and nanoplastics (MNPs) represent one of the most widespread environmental pollutants of the twenty-first century to which all humans are orally exposed. Upon ingestion, MNPs pass harsh biochemical conditions within the gastrointestinal tract, causing a unique protein corona on the MNP surface. Little is known about the digestion-associated protein corona and its impact on the cellular uptake of MNPs. Here, we systematically studied the influence of gastrointestinal digestion on the cellular uptake of neutral and charged polystyrene MNPs using THP-1-derived macrophages.
resultsThe protein corona composition was quantified using LC‒MS-MS-based proteomics, and the cellular uptake of MNPs was determined using flow cytometry and confocal microscopy. Gastrointestinal digestion resulted in a distinct protein corona on MNPs that was retained in serum-containing cell culture medium. Digestion increased the uptake of uncharged MNPs below 500 nm by 4.0-6.1-fold but did not affect the uptake of larger sized or charged MNPs. Forty proteins showed a good correlation between protein abundance and MNP uptake, including coagulation factors, apolipoproteins and vitronectin.
conclusionThis study provides quantitative data on the presence of gastrointestinal proteins on MNPs and relates this to cellular uptake, underpinning the need to include the protein corona in hazard assessment of MNPs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.