Evidence map›Paper›PMID 38310069›Full record

ArticleBritish journal of anaesthesia2024

Enhancing spinal cord stimulation-induced pain inhibition by augmenting endogenous adenosine signalling after nerve injury in rats.

Xiang Cui, Jing Liu, Ankit Uniyal, Qian Xu, Chi Zhang, Guangwu Zhu, Fei Yang, Eellan Sivanesan, Bengt Linderoth, Srinivasa N Raja and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in British journal of anaesthesia, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Xiang CuiDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Jing LiuDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Ankit UniyalDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Qian XuThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, School of Medicine, Baltimore, MD, USA; Howard Hughes Medical Institute, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Chi ZhangDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Guangwu ZhuDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Fei YangDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Eellan SivanesanDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Bengt LinderothDepartment of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden.
Srinivasa N RajaDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA.
Yun GuanDepartment of Anaesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, USA; Department of Neurological Surgery, Johns Hopkins University, School of Medicine, Baltimore, MD, USA. Electronic address: yguan1@jhmi.edu.
Johns Hopkins University · USHoward Hughes Medical Institute · USKarolinska Institutet · SE

Funding

Birth Tissue Products for Non-opioid Treatment of Post-surgical PainR01NS117761 · NINDS · JOHNS HOPKINS UNIVERSITY · PI GUAN, YUN · 2020 to 2024
$2.6M
Mechanistic Study of Pain Inhibition by Activation of Non-nociceptive Afferent FibersR01NS110598 · NINDS · JOHNS HOPKINS UNIVERSITY · PI GUAN, YUN · 2019 to 2024
$2.4M
NINDS NIH HHS R01 NS110598NINDS NIH HHS R01 NS117761
6 · The paper itself

Abstract

backgroundThe mechanisms for spinal cord stimulation (SCS) to alleviate chronic pain are only partially known. We aimed to elucidate the roles of adenosine A1 and A3 receptors (A1R, A3R) in the inhibition of spinal nociceptive transmission by SCS, and further explored whether 2'-deoxycoformycin (dCF), an inhibitor of adenosine deaminase, can potentiate SCS-induced analgesia.

methodsWe used RNAscope and immunoblotting to examine the distributions of adora1 and adora3 expression, and levels of A1R and A3R proteins in the spinal cord of rats after tibial-spared nerve injury (SNI-t). Electrophysiology recording was conducted to examine how adenosine receptor antagonists, virus-mediated adora3 knockdown, and dCF affect SCS-induced inhibition of C-fibre-evoked spinal local field potential (C-LFP).

resultsAdora1 was predominantly expressed in neurones, whereas adora3 is highly expressed in microglial cells in the rat spinal cord. Spinal application of antagonists (100 μl) of A1R (8-cyclopentyl-1,3-dipropylxanthine [DPCPX], 50 μM) and A3R (MRS1523, 200 nM) augmented C-LFP in SNI-t rats (DPCPX: 1.39 [0.18] vs vehicle: 0.98 [0.05], P=0.046; MRS1523: 1.21 [0.07] vs vehicle: 0.91 [0.03], P=0.002). Both drugs also blocked inhibition of C-LFP by SCS. Conversely, dCF (0.1 mM) enhanced SCS-induced C-LFP inhibition (dCF: 0.60 [0.04] vs vehicle: 0.85 [0.02], P<0.001). In the behaviour study, dCF (100 nmol 15 μl

conclusionsSpinal A1R and A3R signalling can exert tonic suppression and also contribute to SCS-induced inhibition of spinal nociceptive transmission after nerve injury. Inhibition of adenosine deaminase may represent a novel adjuvant pharmacotherapy to enhance SCS-induced analgesia.

Indexed as

Adenosine DeaminaseSpinal Cord StimulationAdenosineAnimalsPainRatsSpinal CordAdenosineAdenosine Deaminaseadenosine deaminase inhibitoradenosinergic signallinglocal field potentialneuromodulationneuropathic painspinal cord stimulationspinal nerve injury

Identifiers

PMID38310069
PMCPMC10925891
OpenAlexW4391471178

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.