Evidence map›Paper›PMID 38308742›Full record

ArticleTissue engineering and regenerative medicine2024

α-Gal Nanoparticles in CNS Trauma: II. Immunomodulation Following Spinal Cord Injury (SCI) Improves Functional Outcomes.

Bhavani Gopalakrishnan, Uri Galili, Megan Saenger, Noah J Burket, Wendy Koss, Manjari S Lokender, Kaitlyn M Wolfe, Samantha J Husak, Collin J Stark, Luis Solorio and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering and regenerative medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 1 country.

Bhavani GopalakrishnanCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Uri GaliliDepartment of Medicine, Rush University Medical Center, Chicago, IL, USA.
Megan SaengerCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Noah J BurketCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Wendy KossPurdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN, 47907, USA.
Manjari S LokenderCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Kaitlyn M WolfeCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Samantha J HusakCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Collin J StarkCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Luis SolorioWeldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
Abigail CoxDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN, 47907, USA.
August DunbarCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Riyi ShiCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA.
Jianming LiCenter for Paralysis Research, Purdue University, West Lafayette, IN, 47907, USA. dr.jianming.li@gmail.com.
Purdue University West Lafayette · USRush University Medical Center · US

Funding

A novel enzymatic mechanism for removing neurotoxic aldehydes after rodent spinal cord injuryR21NS115094 · NINDS · PURDUE UNIVERSITY · PI SHI, RIYI · 2019 to 2020
$424k
NINDS NIH HHS 1R21NS115094-01NINDS NIH HHS R21 NS115094
6 · The paper itself

Abstract

backgroundPrevious investigations have shown that local application of nanoparticles presenting the carbohydrate moiety galactose-α-1,3-galactose (α-gal epitopes) enhance wound healing by activating the complement system and recruiting pro-healing macrophages to the injury site. Our companion in vitro paper suggest α-gal epitopes can similarly recruit and polarize human microglia toward a pro-healing phenotype. In this continuation study, we investigate the in vivo implications of α-gal nanoparticle administration directly to the injured spinal cord.

methodsα-Gal knock-out (KO) mice subjected to spinal cord crush were injected either with saline (control) or with α-gal nanoparticles immediately following injury. Animals were assessed longitudinally with neurobehavioral and histological endpoints.

resultsMice injected with α-gal nanoparticles showed increased recruitment of anti-inflammatory macrophages to the injection site in conjunction with increased production of anti-inflammatory markers and a reduction in apoptosis. Further, the treated group showed increased axonal infiltration into the lesion, a reduction in reactive astrocyte populations and increased angiogenesis. These results translated into improved sensorimotor metrics versus the control group.

conclusionsApplication of α-gal nanoparticles after spinal cord injury (SCI) induces a pro-healing inflammatory response resulting in neuroprotection, improved axonal ingrowth into the lesion and enhanced sensorimotor recovery. The data shows α-gal nanoparticles may be a promising avenue for further study in CNS trauma.

Indexed as

NanoparticlesSpinal Cord InjuriesAnimalsAnti-Inflammatory AgentsEpitopesGalactoseHumansImmunomodulationMiceMice, KnockoutAnti-Inflammatory AgentsEpitopesGalactoseAnti-inflammatoryMicrogliaNeuroprotectionSpinal cord injuryα-gal nanoparticles

Identifiers

PMID38308742
PMCPMC10987462
OpenAlexW4391508291

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.