Evidence map›Paper›PMID 38307857›Full record

ArticleNature communications2024

Molecular-level architecture of Chlamydomonas reinhardtii's glycoprotein-rich cell wall.

Alexandre Poulhazan, Alexandre A Arnold, Frederic Mentink-Vigier, Artur Muszyński, Parastoo Azadi, Adnan Halim, Sergey Y Vakhrushev, Hiren Jitendra Joshi, Tuo Wang, Dror E Warschawski and 1 more

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Applications of the MicroalgaeLife (Basel, Switzerland) · 2024
    Review
  11. The MicroalgaeCells · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 4 countries.

Alexandre PoulhazanDepartment of Chemistry, Université du Québec à Montréal, Montreal, QC, H2X 2J6, Canada.
Alexandre A ArnoldDepartment of Chemistry, Université du Québec à Montréal, Montreal, QC, H2X 2J6, Canada.
Frederic Mentink-VigierNational High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, 32310, USA.ORCID 0000-0002-3570-9787
Artur MuszyńskiComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.ORCID 0000-0003-3497-9977
Parastoo AzadiComplex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
Adnan HalimCopenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.
Sergey Y VakhrushevCopenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.ORCID 0000-0002-0418-5765
Hiren Jitendra JoshiCopenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.ORCID 0000-0002-8192-2829
Tuo WangDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA. wangtuo1@msu.edu.ORCID 0000-0002-1801-924X
Dror E WarschawskiLaboratoire des Biomolécules, LBM, CNRS UMR 7203, Sorbonne Université, École Normale Supérieure, PSL University, 75005, Paris, France. dror.warschawski@sorbonne-universite.fr.ORCID 0000-0002-4363-4965
Isabelle MarcotteDepartment of Chemistry, Université du Québec à Montréal, Montreal, QC, H2X 2J6, Canada. marcotte.isabelle@uqam.ca.ORCID 0000-0001-7467-7119
Université du Québec à Montréal · CAUniversity of Copenhagen · DKUniversity of Georgia · USCentre National de la Recherche Scientifique · FRFlorida State University · USMichigan State University · US

Funding

TR&D3-SCHP41GM122698 · NIGMS · FLORIDA STATE UNIVERSITY · PI BREY, WILLIAM W, CROSS, TIMOTHY A · 2017 to 2021
$6.7M
14.1 T magnet with +/-1280 G Field Regulation and Integrated MAS Cryogenic SystemS10OD018519 · OD · FLORIDA STATE UNIVERSITY · PI CROSS, TIMOTHY A · 2014 to 2014
$1.4M
NIGMS NIH HHS P41 GM122698NIH HHS S10 OD018519ODCDC CDC HHS S10 OD018519
6 · The paper itself

Abstract

Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.

Indexed as

ChlamydomonasChlamydomonas reinhardtiiCelluloseCell WallGlycoproteinsWaterCelluloseGlycoproteinsWater

Identifiers

PMID38307857
PMCPMC10837150
OpenAlexW4391476405

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.