Evidence map›Paper›PMID 38306392›Full record

ArticlePLoS neglected tropical diseases2024

Metabolomic landscape of macrophage discloses an anabolic signature of dengue virus infection and antibody-dependent enhancement of viral infection.

Li Xu, Min Li, Jingpu Zhang, Dongxiao Li, Jie Tao, Fuchun Zhang, Xia Jin, Jiahai Lu, Tiefu Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS neglected tropical diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
4.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 9 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Dengue Dilemma: Navigating Cross-Reactivity and Immune Challenges.Current topics in microbiology and immunology · 2026
    Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Li XuScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Min LiScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Jingpu ZhangScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Dongxiao LiMetabo-Profile Biotechnology Company, Shanghai, China.
Jie TaoScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Fuchun ZhangGuangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China.
Xia JinScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Jiahai LuKey Laboratory for Tropical Disease Control, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong, China.
Tiefu LiuScientific Research Center, Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.ORCID 0000-0002-1264-3068
Shanghai Public Health Clinical Center · CNGuangzhou Eighth People's Hospital · CNShanghai CASB Biotechnology (China) · CNSun Yat-sen University · CN

Funding

Guangdong Province Science and TechnologyNational Natural Science Foundation of China
6 · The paper itself

Abstract

Dengue virus (DENV) infection causes dengue fever, the most prevalent arthropod-transmitted viral disease worldwide. Viruses are acellular parasites and obligately rely on host cell machinery for reproduction. Previous studies have indicated metabolomic changes in endothelial cell models and sera of animal models and patients with dengue fever. To probe the immunometabolic mechanism of DENV infection, here, we report the metabolomic landscape of a human macrophage cell model of DENV infection and its antibody-dependent enhancement. DENV infection of THP-1-derived macrophages caused 202 metabolic variants, of which amino acids occupied 23.7%, fatty acids 21.78%, carbohydrates 10.4%, organic acids 13.37%, and carnitines 10.4%. These metabolomic changes indicated an overall anabolic signature, which was characterized by the global exhaustion of amino acids, increases of cellular fatty acids, carbohydrates and pentoses, but decreases of acylcarnitine. Significant activation of metabolic pathways of glycolysis, pentose phosphate, amino acid metabolism, and tricarboxylic acid cycle collectively support the overall anabolism to meet metabolic demands of DENV replication and immune activation by viral infection. Totally 88 of 202 metabolic variants were significantly changed by DENV infection, 36 of which met the statistical standard (P<0.05, VIP>1.5) of differentially expressed metabolites, which were the predominantly decreased variants of acylcarnitine and the increased variants of fatty acids and carbohydrates. Remarkably, 11 differentially expressed metabolites were significantly distinct between DENV only infection and antibody-dependent enhancement of viral infection. Our data suggested that the anabolic activation by DENV infection integrates the viral replication and anti-viral immune activation.

Indexed as

DengueDengue VirusVirus DiseasesAmino AcidsAnimalsAntibody-Dependent EnhancementCarbohydratesCarnitineFatty AcidsHumansMacrophagesVirus ReplicationacylcarnitineAmino AcidsCarbohydratesCarnitineFatty Acids

Identifiers

PMID38306392
PMCPMC10866464
OpenAlexW4391468008

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.