ArticleNature chemical biology2024
Mapping microhabitats of lignocellulose decomposition by a microbial consortium.
Article in Nature chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Mass spectrometry-based human spatial omics: fundamentals, innovations, and applications.Journal of biomedical science · 2026Pooled it
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- The oleaginous yeast Cutaneotrichosporon oleaginosum modifies corn stover alkali lignin.Scientific reports · 2026Article
- Deciphering taxon-specific metabolic shifts during pathogenic fungal infection in leaf-cutter ant fungal gardens.ISME communications · 2026Article
- spammR: an R package designed for analysis and integration of spatial multi-omic measurements.Bioinformatics advances · 2026Article
- You are what your fungus eats: diet shapes the microbial garden of a fungus-growing ant.NPJ biofilms and microbiomes · 2025Article
- spammR: an R package designed for analysis and integration of spatial multi-omic measurements.bioRxiv : the preprint server for biology · 2025Article
- The Future of a Myriad of Accelerated Biodiscoveries Lies in AI-Powered Mass Spectrometry and Multiomics Integration.Journal of mass spectrometry : JMS · 2025Article
- Harnessing Nutritional Niches to Explore Fungus-Animal Symbioses.Ecology letters · 2025Review
- Spatial top-down proteomics for the functional characterization of human kidney.Clinical proteomics · 2025Article
- Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution.Nature communications · 2025Article
- Challenges in Spatial Metabolomics and Proteomics for Functional Tissue Unit and Single-Cell Resolution.Seminars in nephrology · 2024Review
- Spatial top-down proteomics for the functional characterization of human kidney.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
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Authors and funding
24 authors at 2 institutions in 2 countries.
Funding
Abstract
The leaf-cutter ant fungal garden ecosystem is a naturally evolved model system for efficient plant biomass degradation. Degradation processes mediated by the symbiotic fungus Leucoagaricus gongylophorus are difficult to characterize due to dynamic metabolisms and spatial complexity of the system. Herein, we performed microscale imaging across 12-µm-thick adjacent sections of Atta cephalotes fungal gardens and applied a metabolome-informed proteome imaging approach to map lignin degradation. This approach combines two spatial multiomics mass spectrometry modalities that enabled us to visualize colocalized metabolites and proteins across and through the fungal garden. Spatially profiled metabolites revealed an accumulation of lignin-related products, outlining morphologically unique lignin microhabitats. Metaproteomic analyses of these microhabitats revealed carbohydrate-degrading enzymes, indicating a prominent fungal role in lignocellulose decomposition. Integration of metabolome-informed proteome imaging data provides a comprehensive view of underlying biological pathways to inform our understanding of metabolic fungal pathways in plant matter degradation within the micrometer-scale environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.