ArticlePLoS biology2024
Cellular arrangement impacts metabolic activity and antibiotic tolerance in Pseudomonas aeruginosa biofilms.
Article in PLoS biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Co-Expression of PA0290 and PelD EnhancesMicroorganisms · 2026Article
- The power of ten: report from the 10th American Society for Microbiology Conference on Biofilms.Journal of bacteriology · 2026Article
- Biological and clinical significance of iron inJournal of bacteriology · 2026Review
- ClearScope: A Fully Integrated Light-Sheet Theta Microscope for Sub-Micron-Resolution Imaging Without Lateral Size Constraints.Journal of imaging · 2026Article
- Microbial Interactions Shape Spatial Organisation and Transcriptional Responses in a Model Mixed-Species Biofilm.Microbial ecology · 2026Article
- Sculpting Microbial Microenvironments: Spatiotemporal Control via Programmable Electrochemical Gradients.Journal of the American Chemical Society · 2026Review
- Investigating Salmonella biofilm responses to antibiotic treatment using optical photothermal infrared spectroscopy.Communications biology · 2026Article
- Cell-to-Cell and Patient-to-Patient Variability in Antimicrobial Resistance.Microorganisms · 2025Review
- Article
- Single-cell lysis patterns morphogenesis of eDNA in the matrix ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A biofilm-targeting lipo-peptoid to treatBiofilm · 2025Article
- Primary and Re-exposure effects of D-enantiomeric peptide on metabolism, diversity, and composition of oral biofilms at different stages of recovery.Bioactive materials · 2025Article
- Spatiotemporal development of expanding bacterial colonies driven by emergent mechanical constraints and nutrient gradients.Nature communications · 2025Article
- Analysis of biofilm assembly by large area automated AFM.NPJ biofilms and microbiomes · 2025Article
- Oxygen microenvironments inMicrobiology (Reading, England) · 2025Article
- Individual-based modeling unravels spatial and social interactions in bacterial communities.The ISME journal · 2025Review
- Biofilms as more than the sum of their parts: lessons from developmental biology.Current opinion in microbiology · 2024Review
- The L-lactate dehydrogenases ofmBio · 2024Article
- Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples.Nature biomedical engineering · 2024Article
- Article
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13 authors.
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Abstract
Cells must access resources to survive, and the anatomy of multicellular structures influences this access. In diverse multicellular eukaryotes, resources are provided by internal conduits that allow substances to travel more readily through tissue than they would via diffusion. Microbes growing in multicellular structures, called biofilms, are also affected by differential access to resources and we hypothesized that this is influenced by the physical arrangement of the cells. In this study, we examined the microanatomy of biofilms formed by the pathogenic bacterium Pseudomonas aeruginosa and discovered that clonal cells form striations that are packed lengthwise across most of a mature biofilm's depth. We identified mutants, including those defective in pilus function and in O-antigen attachment, that show alterations to this lengthwise packing phenotype. Consistent with the notion that cellular arrangement affects access to resources within the biofilm, we found that while the wild type shows even distribution of tested substrates across depth, the mutants show accumulation of substrates at the biofilm boundaries. Furthermore, we found that altered cellular arrangement within biofilms affects the localization of metabolic activity, the survival of resident cells, and the susceptibility of subpopulations to antibiotic treatment. Our observations provide insight into cellular features that determine biofilm microanatomy, with consequences for physiological differentiation and drug sensitivity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.