ArticleScience translational medicine2024
Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding.
Article in Science translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Resolving endogenous protein organization in cells with nanometer resolution.Nature communications · 2026Article
- Single-Step Expansion SMLM Enables Molecular-Resolution Imaging in Cells and Isolated Proteins.Chemical & biomedical imaging · 2026Article
- Article
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Principles ofbioRxiv : the preprint server for biology · 2026Article
- Visualising viral interactions and mechanisms at the nanoscale with expansion microscopy.Npj viruses · 2026Review
- Expansion microscopy of banked brain tissue.Free neuropathology · 2026Article
- A transcriptomic atlas of astrocyte heterogeneity across space and time in mouse and marmoset.Neuron · 2025Article
- Review
- Serial multiomics uncovers anti-glioblastoma responses not evident by routine clinical analyses.Science translational medicine · 2025Article
- Astrocyte regional specialization is shaped by postnatal development.bioRxiv : the preprint server for biology · 2025Article
- BOOST: a robust ten-fold expansion method on hour-scale.Nature communications · 2025Article
- Multiplexed expansion revealing for imaging multiprotein nanostructures in healthy and diseased brain.Nature communications · 2024Article
- Single-shot 20-fold expansion microscopy.Nature methods · 2024Article
- Subdiffraction Imaging of Cleared and Expanded Large-Scale Tissues.Chemical & biomedical imaging · 2024Review
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Authors and funding
13 authors.
Funding
Abstract
Proteins are densely packed in cells and tissues, where they form complex nanostructures. Expansion microscopy (ExM) variants have been used to separate proteins from each other in preserved biospecimens, improving antibody access to epitopes. Here, we present an ExM variant, decrowding expansion pathology (dExPath), that can expand proteins away from each other in human brain pathology specimens, including formalin-fixed paraffin-embedded (FFPE) clinical specimens. Immunostaining of dExPath-expanded specimens reveals, with nanoscale precision, previously unobserved cellular structures, as well as more continuous patterns of staining. This enhanced molecular staining results in observation of previously invisible disease marker-positive cell populations in human glioma specimens, with potential implications for tumor aggressiveness. dExPath results in improved fluorescence signals even as it eliminates lipofuscin-associated autofluorescence. Thus, this form of expansion-mediated protein decrowding may, through improved epitope access for antibodies, render immunohistochemistry more powerful in clinical science and, perhaps, diagnosis.
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