Evidence map›Paper›PMID 38293991›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2024

[LncRNA SOX2OT enhances 5-fluorouracil resistance of cholangiocarcinoma cells by promoting autophagy

C Xin, X Wang, X Li, Y Chen, X Wang, J Ning, S Yang, Z Wang

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

C XinDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
X WangDepartment of Anesthesiology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
X LiDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Y ChenDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
X WangDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
J NingDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
S YangDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Z WangDepartment of Gastroenterology, Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Affiliated Hospital of Southwest Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the role of SIRT1/autophagy pathway in mediating the regulatory effect of lncRNA SOX2OT on 5-fluorouracil (5-FU) resistance in cholangiocarcinoma cells.

methodsHCCC-9810 cells were used to construct a 5-FU-resistant cell model (HCCC-9810/5-FU cells), and the expression levels of lncRNA SOX2OT and SIRT1 mRNA and the protein expressions of SIRT1, Beclin1, LC3 and P62 were detected with qRT-PCR and Western blotting. The effects of transfection with a SOX2OT mimic on drug resistance and cell migration of HCCC-9810/5-FU cells were detected using CCK-8 assay and wound healing assay, and the changes in expressions of SOX2OT, SIRT1, Beclin1, LC3 and P62 were detected. Rescue experiment was performed by co-transfection of HCCC-9810/5-FU cells with both a SOX2OT-overexpressing plasmid and si-SIRT1 to confirm the role of SIRT1 in SOX2OT-mediated regulation of 5-FU resistance. A RNA pulldown assay was used to verify the targeted binding between SOX2OT and SIRT1.

resultsThe proliferation of HCCC-9810 cells was significantly inhibited after treatment with different concentrations of 5-FU (

conclusionLncRNA SOX2OT enhances 5-FU resistance in HCCC-9810 cells by promoting autophagy through up-regulating SIRT1 expression.

Indexed as

CholangiocarcinomaRNA, Long NoncodingAutophagyBeclin-1Cell Line, TumorFluorouracilHumansRNA, MessengerSirtuin 1Beclin-1Fluorouracillong non-coding RNA Sox2ot, humanRNA, Long NoncodingRNA, MessengerSIRT1 protein, humanSirtuin 15-FU resistantautophagycholangiocarcinoma cellsLncRNA SOX2OTSIRT1

Identifiers

PMID38293991
PMCPMC10878893
OpenAlexW4394760396

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.