Evidence map›Paper›PMID 38289715›Full record

ArticleActa crystallographica. Section D, Structural biology2024

Deciphering the crystal structure of a novel nanobody against the NEIL1 DNA glycosylase.

Marlo K Thompson, Nidhi Sharma, Andrea Thorn, Aishwarya Prakash

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section D, Structural biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marlo K ThompsonMitchell Cancer Institute, University of South Alabama Health, 1660 Springhill Avenue, Mobile, AL 36604, USA.
Nidhi SharmaMitchell Cancer Institute, University of South Alabama Health, 1660 Springhill Avenue, Mobile, AL 36604, USA.
Andrea ThornInstitut für Nanostruktur und Festkörperphysik, Universität Hamburg, Hamburg, Germany.ORCID 0000-0003-4503-4258
Aishwarya PrakashMitchell Cancer Institute, University of South Alabama Health, 1660 Springhill Avenue, Mobile, AL 36604, USA.

Funding

Repair of Environmentally Induced Mitochondrial DNA DamageR01ES030084 · NIEHS · UNIVERSITY OF SOUTH ALABAMA · PI PRAKASH, AISHWARYA · 2019 to 2023
$2.3M
NIEHS NIH HHS R01 ES030084
6 · The paper itself

Abstract

Nanobodies (VHHs) are single-domain antibodies with three antigenic CDR regions and are used in diverse scientific applications. Here, an ∼14 kDa nanobody (A5) specific for the endonuclease VIII (Nei)-like 1 or NEIL1 DNA glycosylase involved in the first step of the base-excision repair pathway was crystallized and its structure was determined to 2.1 Å resolution. The crystals posed challenges due to potential twinning and anisotropic diffraction. Despite inconclusive twinning indicators, reprocessing in an orthorhombic setting and molecular replacement in space group P2

Indexed as

DNA GlycosylasesDNA GlycosylasesnanobodiesNEIL1 DNA glycosylaseorthorhombic space grouppseudo-merohedral twinningX-ray crystallography

Identifiers

PMID38289715
PMCPMC10836396

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.