Evidence map›Paper›PMID 38289481›Full record

ArticleBiomedical microdevices2024

Microneedle patch-based enzyme-linked immunosorbent assay to quantify protein biomarkers of tuberculosis.

Youngeun Kim, Mary Beth Lewis, Jihyun Hwang, Zheyu Wang, Rohit Gupta, Yuxiong Liu, Tuhina Gupta, James P Barber, Srikanth Singamaneni, Fred Quinn and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical microdevices, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Youngeun KimSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Mary Beth LewisDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA.
Jihyun HwangSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Zheyu WangDepartment of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
Rohit GuptaDepartment of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
Yuxiong LiuDepartment of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
Tuhina GuptaDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA.
James P BarberDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA.
Srikanth SingamaneniDepartment of Mechanical Engineering and Materials Science, Washington University in St. Louis, St. Louis, MO, 63130, USA.
Fred QuinnDepartment of Infectious Diseases, University of Georgia, Athens, GA, 30602, USA.
Mark R PrausnitzSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA. prausnitz@gatech.edu.
University of Georgia · USWashington University in St. Louis · USGeorgia Institute of Technology · US

Funding

Emory/Georgia TB Research Advancement Center (TRAC)P30AI168386 · NIAID · EMORY UNIVERSITY · PI Jeffrey M Collins · 2022 to 2026
$5.8M
Detection of early-stage, active tuberculosis infection and disease progression during treatment using a rapid, inexpensive and simple-to-use microneedle patch diagnostic platformR21EB025499 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI PRAUSNITZ, MARK R., QUINN, FREDERICK D · 2018 to 2019
$519k
NIAID NIH HHS P30 AI168386NIBIB NIH HHS R21 EB025499NIH HHS EB025499
6 · The paper itself

Abstract

There is a clinical need for differential diagnosis of the latent versus active stages of tuberculosis (TB) disease by a simple-to-administer test. Alpha-crystallin (Acr) and early secretory antigenic target-6 (ESAT-6) are protein biomarkers associated with the latent and active stages of TB, respectively, and could be used for differential diagnosis. We therefore developed a microneedle patch (MNP) designed for application to the skin to quantify Acr and ESAT-6 in dermal interstitial fluid by enzyme-linked immunosorbent assay (ELISA). We fabricated mechanically strong microneedles made of polystyrene and coated them with capture antibodies against Acr and ESAT-6. We then optimized assay sensitivity to achieve a limit of detection of 750 pg/ml and 3,020 pg/ml for Acr and ESAT-6, respectively. This study demonstrates the feasibility of an MNP-based ELISA for differential diagnosis of latent TB disease.

Indexed as

TuberculosisAntibodiesBiological TransportBiomarkersEnzyme-Linked Immunosorbent AssayHumansAntibodiesBiomarkersELISA assayMicroneedle patchProtein biomarkersSkin interstitial fluidTuberculosis

Identifiers

PMID38289481
PMCPMC13205957
OpenAlexW4391360305

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.