Evidence map›Paper›PMID 38287974›Full record

ArticleFrontiers in cellular and infection microbiology2023

A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen-specific T cells.

Kathrin Balz, Magali Grange, Uta Pegel, Zain A Karamya, Marielle Mello, Xiaoying Zhou, Thilo Berger, Konstantin Bloch, Diane Dunham, Sharon Chinthrajah and 3 more

Open access · goldAbstract read
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In one paragraph

Article in Frontiers in cellular and infection microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Kathrin Balz *Institute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
Magali Grange *Centre d'Immunophénomique Centre d'Immunophénomique (CIPHE), Aix Marseille Université, INSERM, CNRS Philipps-Universität Marburg (UMR), Marseille, France.
Uta PegelInstitute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
Zain A KaramyaInstitute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
Marielle MelloCentre d'Immunophénomique Centre d'Immunophénomique (CIPHE), Aix Marseille Université, INSERM, CNRS Philipps-Universität Marburg (UMR), Marseille, France.
Xiaoying ZhouDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United States.
Thilo BergerInstitute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
Konstantin BlochInstitute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
Diane DunhamSean N Parker Center for Allergy and Asthma Research at Stanford University, Stanford, CA, United States.
Sharon ChinthrajahSean N Parker Center for Allergy and Asthma Research at Stanford University, Stanford, CA, United States.
Kari Nadeau *Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, United States.
Hervé Luche *Centre d'Immunophénomique Centre d'Immunophénomique (CIPHE), Aix Marseille Université, INSERM, CNRS Philipps-Universität Marburg (UMR), Marseille, France.
Chrysanthi Skevaki *Institute of Laboratory Medicine, Universities of Giessen and Marburg Lung Center (UGMLC), Philipps University Marburg, German Center for Lung Research (DZL), Marburg, Germany.
German Center for Lung Research · DECentre National de la Recherche Scientifique · FRHarvard University · USStanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the development of vaccines and therapeutics. We aimed on optimizing immunophenotyping of T cells after antigen stimulation by improving staining procedures for flow and mass cytometry. Our method can be used for primary cells of both mouse and human origin for the detection of low-frequency T-cell response using a dual-barcoding system for individual samples and conditions. First, live-cell barcoding was performed using anti-CD45 antibodies prior to an

Indexed as

AntigensT-LymphocytesAnimalsCD8-Positive T-LymphocytesFlow CytometryHumansImmunophenotypingMiceStaining and LabelingAntigensCyTOFmethodsample barcodingT-cell activationT-cell stimulation assay

Identifiers

PMID38287974
PMCPMC10822892
OpenAlexW4390883996

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.