Evidence map›Paper›PMID 38285390›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription.

Xue Jiang, Kekely Bruno Attiogbe, Yating Guo, Xiaoyun Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xue JiangCollege of Plant Protection, Northeast Agricultural University, Harbin, China.
Kekely Bruno AttiogbeCollege of Plant Protection, Northeast Agricultural University, Harbin, China.
Yating GuoCollege of Plant Protection, Northeast Agricultural University, Harbin, China.
Xiaoyun WuCollege of Plant Protection, Northeast Agricultural University, Harbin, China. xiaoyun.wu@neau.edu.cn.
Northeast Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large-scale and cost-less production of double-stranded RNA (dsRNA) is the basis for the widespread application of dsRNA in agriculture. Bidirectional transcription of target sequence in RNase III-deficient Escherichia coli strain HT115 (DE3) is an efficient way to produce large amounts of dsRNA. Here, we present a detailed method for the production of dsRNA by bidirectional transcription in E. coli from vector construction, induction of expression by isopropylthio-β-galactoside (IPTG), and purification of dsRNA from E. coli.

Indexed as

Escherichia coliRNA, Double-StrandedAgricultureProkaryotic CellsPromoter Regions, GeneticRNA, Double-StrandedBidirectional transcriptiondsRNAProkaryotic promoterRNase III-deficientT7

Identifiers

PMID38285390
OpenAlexW4391315671

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.