Evidence map›Paper›PMID 38285354›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Stereological Approach to Quantify Immunohistochemical Staining in Zebrafish Larvae.

Dércia Santos, Sandra M Monteiro, Ana Luzio

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Dércia SantosCentre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal.
Sandra M MonteiroCentre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal.
Ana LuzioCentre for the Research and Technology of Agro-Environmental and Biological Sciences, CITAB and Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-food Production, Quinta de Prados, Vila Real, Portugal. aluzio@utad.pt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunohistochemistry (IHC) is a powerful research tool to localize specific antigens in whole-mount or tissue sections of embryos with labeled antibodies based on antigen-antibody interactions. Stereological methods are nowadays an essential tool to quantify cells or other types of structures in an unbiased and reproducible manner. In this chapter, a general protocol for a stereological estimation of the relative volume density of each structural component (Vv), which can be applied to any organ/structural component, will be described.

Indexed as

PerciformesZebrafishAnimalsAntibodiesLarvaStaining and LabelingAntibodiesAbsolute volume (V)Histological techniquesPoint-countingRelative volume density (Vv)

Identifiers

PMID38285354

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.